Assignment of the human aggrecan gene (AGC1) to 15q26 using fluorescence in situ hybridization analysis
| Autor: | Julie R. Korenberg, Judy Grover, Kurt J. Doege, Peter J. Roughley, Xiao N. Chen |
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| Rok vydání: | 1993 |
| Předmět: |
Molecular Sequence Data
Biology Exon Gene mapping Genetics medicine Humans Lectins C-Type Aggrecans Peptide sequence Gene Aggrecan In Situ Hybridization Fluorescence Chromosomes Human Pair 15 Extracellular Matrix Proteins medicine.diagnostic_test Base Sequence Infant Newborn Chromosome Chromosome Mapping DNA Molecular biology Chromosome Band Genes Proteoglycans Fluorescence in situ hybridization |
| Zdroj: | Genomics. 16(2) |
| ISSN: | 0888-7543 |
| Popis: | The large aggregating proteoglycan aggrecan is a major structural component of the extracellular matrix of articular cartilage. Recent cDNA cloning of the human aggrecan gene (AGC1) reveals a core protein of at least 2316 amino acids characterized by several distinct structural domains. Two globular domains, termed G1 and G2, are present at the amino terminus of the molecule and a third, termed G3, is present at the carboxy terminus. The G1 domain is homologous in structure to the cartilage link protein and accounts for the aggregating potential of aggrecan through its ability to interact with hyaluronic acid. The aggrecan gene is known to consist of 15 exons, with each exon encoding a distinct functional region of the mature protein. However, while the link protein gene is known to reside on chromosome 5 in the human, the location of the aggrecan gene is currently undetermined in any species. The probe (pAGG2) for the aggrecan gene was mapped on chromosome band 15q26, most likely in the subregion of 15q26.1, using fluorescence in situ hybridization. Clear signals were noted on both chromatids of chromosome band 15q26 in over 80% of the 300 metaphase cells examined in three independent experiments using pAGG2. No othermore » sites of hybridization were noted on both chromatids of any other chromosome band. The precise band location was identified by using chromsomes of about 650 bands and employing fluorescence reverse banding with chromomycin A3 and distamycin. 14 refs., 1 fig.« less |
| Databáze: | OpenAIRE |
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