Transferrin Enzyme Immunoassay for Quantitative Monitoring of Blood Contamination in Saliva
| Autor: | Eve B. Schwartz, Douglas A. Granger |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
chemistry.chemical_classification
Saliva Urinalysis medicine.diagnostic_test business.industry Biochemistry (medical) Clinical Biochemistry Transferrin Physiology Contamination Immunoenzyme Techniques Blood medicine.anatomical_structure chemistry Immunoassay Immunology medicine Humans Hemoglobin Oral mucosa business Hormone |
| Zdroj: | Clinical Chemistry. 50:654-656 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1373/clinchem.2003.028266 |
| Popis: | When blood components are present in the oral mucosa, quantitative estimates of salivary hormone concentrations may be compromised (1). Blood and its components can leak into the oral mucosa as a result of micro injuries such as burns or abrasions. The probability of blood leakage is increased with poor oral health (i.e., periodontal disease), certain infectious diseases (e.g., HIV) (2), and behavior known to influence oral health negatively (e.g., smoking) (3). Few studies, however, have investigated methods to determine blood contamination in saliva. The Hemastix® Reagent Strip for Urinalysis has been used to test for blood in saliva (4)(5). This method detects the pseudoperoxidase activity of hemoglobin; thus, endogenous salivary peroxidases produce false-positive results for hemoglobin. To provide a quantitative measure of blood contamination in saliva, we developed an enzyme immunoassay for transferrin, which is present in very small amounts ( 1000 mg/L). We expect that serum and salivary concentrations of transferrin are not correlated and that transferrin, with a formula weight of 76 000 and radius of 5 nm, would reach saliva in high concentrations only when the barrier between blood and saliva is compromised. Saliva (by passive drool) and serum samples were collected without stimulation simultaneously from 40 young adults. The Pennsylvania State University Institutional Review Board approved all procedures, and informed consent was obtained. All samples were stored at −40 °C. All samples were thawed, vortex-mixed, and centrifuged at 1500 g for 15 min before assay for serum transferrin, salivary transferrin, or testing by Hemastix Reagent … |
| Databáze: | OpenAIRE |
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