Effect of colibacillosis on the immune response to a rabbit viral haemorrhagic disease vaccine
| Autor: | Hanaa S. Ali, Abdelfattah H. Eladl, Reham A. El-Shafei, Mohamed A. Saif, Verginia M. Farag, Rasha M. Elkenany, Marwa M. Mona, Mona Elsayed |
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| Rok vydání: | 2019 |
| Předmět: |
Necrosis
Hemorrhagic Disease Virus Rabbit medicine.medical_treatment Biology Microbiology Median lethal dose Virus Rabbit haemorrhagic disease 03 medical and health sciences Immune system medicine Animals Escherichia coli Infections 030304 developmental biology Caliciviridae Infections 0303 health sciences General Veterinary 030306 microbiology Mortality rate Vaccination Immunosuppression Viral Vaccines General Medicine biology.organism_classification Gene Expression Regulation Immunology Cytokines Rabbits medicine.symptom |
| Zdroj: | Veterinary microbiology. 238 |
| ISSN: | 1873-2542 |
| Popis: | Viral haemorrhagic disease (VHD) and colibacillosis are common diseases in rabbits that cause economic losses worldwide. The effect of colibacillosis on the immune response of vaccinated rabbits against rabbit haemorrhagic disease virus (RHDV) was studied. Four groups (G1-G4) were included. G1 was the negative control group; G2 was the RHDV vaccine group; G3 was the E. coli-infected group; and G4 was the E. coli-infected + RHDV vaccine group. The E. coli infection and RHDV vaccination were simultaneously performed, with another previous infection, 3 days before vaccination. At 28 days post-vaccination (PV), the rabbits (G2-G4) were challenged intramuscularly with 0.5 ml of RHDV at a dose of 103 50% median lethal dose (LD50)/rabbit. The rabbits were observed for clinical signs, body weight gain and mortality rates. Tissue, blood, serum, and faecal samples and rectal swabs were collected at 3, 5, 7, 14, 21 and 28 days PV. Significant clinical signs and mortality and a decrease in BW were observed in the infected + RHDV vaccine group. On the 3rd day post-infection (PI), compared with all the other groups, the vaccinated group (G2) had significantly upregulated hepatic tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels; however, the infected + RHDV vaccine group had significantly higher intestinal levels of TNF-α and IL-6 than the other groups. Furthermore, E. coli infection in vaccinated rabbits led to immunosuppression, as shown by significant decreases (P |
| Databáze: | OpenAIRE |
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