A diagnostic method for herpes simplex keratitis by simultaneous measurement of viral DNA and virus-specific secretory IgA in tears: an evaluation
| Autor: | Masao Matsubara, Tohru Sakimoto, Etsuko Takamura, Mitsuru Sawa, Yuko Kamei, Noriko Inada, Jun Shoji |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
Simplexvirus food.ingredient Corneal Stroma viruses Enzyme-Linked Immunosorbent Assay HSL and HSV Antibodies Viral Real-Time Polymerase Chain Reaction medicine.disease_cause Virus Keratitis law.invention 03 medical and health sciences 0302 clinical medicine food law medicine Humans Prospective Studies Fluorescent Antibody Technique Indirect Polymerase chain reaction business.industry General Medicine Middle Aged medicine.disease eye diseases Ophthalmology Real-time polymerase chain reaction Herpes simplex virus Tears DNA Viral Immunoglobulin A Secretory Immunology Keratitis Herpetic 030221 ophthalmology & optometry Female sense organs business 030217 neurology & neurosurgery |
| Zdroj: | Japanese Journal of Ophthalmology. 60:294-301 |
| ISSN: | 1613-2246 0021-5155 |
| Popis: | We performed simultaneous measurement of herpes simplex virus (HSV) DNA by real-time polymerase chain reaction (real-time PCR) and of HSV-specific secretory IgA antibody (HSV-sIgA) by enzyme-linked immunosorbent assay (ELISA) in tears obtained using Schirmer strips in order to investigate its diagnostic efficacy for herpes simplex keratitis (HSK). A total of 59 affected eyes from 59 patients with clinically suspected HSK (HSK group) and 23 eyes from 23 healthy volunteers (control group) were enrolled in this study. The HSK group was divided into five subgroups: dendritic/geographic keratitis, disciform keratitis, necrotizing keratitis, atypical keratitis, and others. The tear samples were taken using Schirmer strips to determine the HSV DNA and HSV-sIgA levels. The overall sensitivity and specificity were 55.8 and 100 % for HSV DNA and 49.2 and 82.6 % for HSV-sIgA. The HSV DNA levels in the disciform keratitis subgroup (median, 3.1 × 102 copies/sample) were significantly lower than those in the dendritic/geographic keratitis subgroup (median, 2.3 × 104 copies/sample) (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink