Cucumber mosaic virus as a presentation system for a double hepatitis C virus-derived epitope
| Autor: | Isabella Stella, Giuseppina Piazzolla, Salvatore Antonaci, A. Natilla, Cosimo Tortorella, Pasquale Piazzolla, Mauro Lapelosa, Antonella Vitti, Maria Nuzzaci |
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| Rok vydání: | 2006 |
| Předmět: |
Models
Molecular Viral Hepatitis Vaccines Hepatitis C virus Genetic Vectors Molecular Sequence Data Hepacivirus medicine.disease_cause Lymphocyte Activation Cucumovirus Virus Microbiology Cucumber mosaic virus Epitopes Interferon-gamma Antibody Specificity Virology Plant virus medicine Tobacco mosaic virus Humans Amino Acid Sequence biology Mimotope Immune Sera virus diseases General Medicine biology.organism_classification Plants Genetically Modified Hepatitis C Recombinant Proteins Alfalfa mosaic virus Leukocytes Mononuclear Capsid Proteins Peptides Sequence Alignment Reassortant Viruses |
| Zdroj: | Archives of virology. 152(5) |
| ISSN: | 0304-8608 |
| Popis: | Chimeric plant viruses are emerging as promising vectors for use in innovative vaccination strategies. In this context, cucumber mosaic virus (CMV) has proven to be a suitable carrier of the hepatitis C virus (HCV)-derived R9 mimotope. In the present work, a new chimeric CMV, expressing on its surface the HCV-derived R10 mimotope, was produced but lost the insert after the first passage on tobacco. A comparative analysis between R10- and R9-CMV properties indicated that R9-CMV stability was related to structural features typical of the foreign insert. Thus, in order to combine high virus viability with strong immuno-stimulating activity, we doubled R9 copies on each of the 180 coat protein (CP) subunits of CMV. One of the chimeras produced by this approach (2R9-CMV) was shown to systemically infect the host, stably maintaining both inserts. Notably, it was strongly recognized by sera of HCV-infected patients and, as compared with R9-CMV, displayed an enhanced ability to stimulate lymphocyte IFN-gamma production. The high immunogen levels achievable in plants or fruits infected with 2R9-CMV suggest that this chimeric form of CMV may be useful in the development of oral vaccines against HCV. |
| Databáze: | OpenAIRE |
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