High resolution gel chromatography of proteins
| Autor: | Burkhardt Dahlmann, Lothar Kuehn, Malte Rutschmann, Hans Reinauer |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Gel electrophoresis
Chromatography Resolution (mass spectrometry) Sodium Biophysics Analytical chemistry chemistry.chemical_element High resolution Proteins Dextrans Cell Biology Fractionation Biochemistry Complete resolution Gel permeation chromatography Molecular Weight chemistry Chromatography Gel Molecular Biology Gels |
| Zdroj: | Analytical biochemistry. 124(1) |
| ISSN: | 0003-2697 |
| Popis: | The protein fractionation pattern on three different gels—Sephadex G-75 superfine, Ultrogel AcA 54, and Bio-Gel P-100 minus 400 mesh—has been compared. Analysis of the column eluates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis demonstrated Bio-Gel P-100 to be a gel with very high separation efficiency, allowing complete resolution of proteins differing in M r by as little as 5000. The method is simple and inexpensive and allows the investigator to perform protein fractionations with resolution similar to that possible by high-performance liquid chromatography, but at a preparative scale and with little risk of protein denaturation. |
| Databáze: | OpenAIRE |
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