Physico-chemical characterization and tissue healing changes by Hancornia speciosa Gomes latex biomembrane

Autor: Marco Andrey Cipriani Frade, Bruna Cambraia Garms, Marcio Antoniassi, Luisiane de Avila Santana, Monica Yonashiro Marcelino, Giovana Sant'Ana Pegorin, Ana Laura Destro Chagas, Marcel Nani Leite, Rondinelli Donizetti Herculano
Přispěvatelé: Universidade Estadual Paulista (Unesp), Universidade de São Paulo (USP), University of Brasília (UnB), The University of Queensland
Rok vydání: 2020
Předmět:
Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 1552-4981
Popis: Made available in DSpace on 2021-06-25T10:45:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-07-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Skin wounds have been a public health concern of high frequency, in addition to requiring intensive and expensive care. The natural rubber latex (NRL) from Hancornia speciosa Gomes has been used to treat many problems in traditional medicine and also present healing properties, antifungal and anti-inflammatory activity and antinociceptive effects. The purpose of this study was to characterize the new biomembrane from the NRL of H. speciosa (HS) by Fourier transform infrared (FTIR) and mechanical strength test and to investigate its biological properties by the cytotoxicity assay and in vivo healing activity. The results showed that the HS biomembrane exhibited characteristic bands of the main component cis-1,4-polyisoprene. Besides, its Young modulus was close to human skin with adhesive-compatible mechanical characteristics. The cytotoxicity assays revealed that the HS biomembrane was not toxic to fibroblast cells neither using agar diffusion test nor MTT assay. Furthermore, the HS biomembrane stimulated the inflammatory cells and the angiogenesis, increased significantly the collagenesis and improved the quality of heal until the remodeling phase induced by implants in mice. Thus, this biomembrane has proven to be a safe and biocompatible biomaterial with healing potential, becoming an effective and low-cost alternative for the treatment of skin wounds. Department of Biochemistry and Chemical Technology São Paulo State University (UNESP) Institute of Chemistry Department of Biotechnology and Bioprocesses Engineering São Paulo State University (UNESP) School of Pharmaceutical Sciences Division of Dermatolgoy of Department of Internal Medicine Ribeirão Preto Medical School at São Paulo University (USP), Av. Bandeirantes 3900 Faculty of Ceilândia University of Brasília (UnB) School of Chemistry and Molecular Biosciences The University of Queensland Department of Biochemistry and Chemical Technology São Paulo State University (UNESP) Institute of Chemistry Department of Biotechnology and Bioprocesses Engineering São Paulo State University (UNESP) School of Pharmaceutical Sciences FAPESP: 2009/09355-0 FAPESP: 2016/16437-7 FAPESP: 2017/19603-8 CNPq: 470261/2012
Databáze: OpenAIRE
Externí odkaz: