Quantification of C-ERB-B2 gene amplification in breast cancer cells using fluorescence in situ hybridization and digital image analysis
| Autor: | Carmen López-Fernández, Ismael Buñot, José Luís Fernández, Vicente J. Goyanes, Jaime Gosálvez |
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| Rok vydání: | 1996 |
| Předmět: |
Cancer Research
medicine.diagnostic_test Carcinoma Ductal Breast Gene Amplification Breast Neoplasms Image processing Genes erbB-2 Biology Fluorescence Molecular biology Digital image Gene duplication Digital image analysis Image Processing Computer-Assisted Genetics medicine Humans Female Copy-number variation Breast cancer cells Molecular Biology In Situ Hybridization Fluorescence Fluorescence in situ hybridization |
| Zdroj: | Cancer Genetics and Cytogenetics. 86:18-21 |
| ISSN: | 0165-4608 |
| DOI: | 10.1016/0165-4608(95)00176-x |
| Popis: | Fluorescence in situ hybridization (FISH) allows detection of the intercellular heterogeneity of C-ERB-B 2 gene amplification in uncultured breast cancer cells. Nevertheless, because high levels of amplification result in coalescence of signals, direct microscopy quantification is restricted to cells with low levels of amplification or with dispersed signals. A methodology of digital image analysis, using surface and grey-level FISH signals as parameters that permit a rapid, objective, and accurate estimation of gene copy number, is presented. This procedure is independent of the signal overlapping and results in a more accurate quantification and characterization of tumor cell heterogeneity. |
| Databáze: | OpenAIRE |
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