Synthesis and DNA-binding properties of novel DNA cyclo-intercalators containing purine–glucuronic acid hybrids
| Autor: | Shaopeng Chen, Sumei Ren, Ming-Jing Li, Rilei Yu, Xueting Wang, Tao Jiang, Renshuai Zhang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Purine
Stereochemistry Intercalation (chemistry) 010402 general chemistry 01 natural sciences Biochemistry Fluorescence spectroscopy Analytical Chemistry chemistry.chemical_compound Glucuronic Acid Animals Spectral data Binding Sites 010405 organic chemistry Chemistry Organic Chemistry Binding properties DNA General Medicine Glucuronic acid Intercalating Agents 0104 chemical sciences Molecular Docking Simulation Spectrometry Fluorescence Purines Docking (molecular) Cattle Glycoconjugates |
| Zdroj: | Carbohydrate Research. 429:48-53 |
| ISSN: | 0008-6215 |
| DOI: | 10.1016/j.carres.2016.04.011 |
| Popis: | Novel DNA cyclo-intercalators, which incorporated two intercalator subunits linked by two bridges, were synthesized. Binding of the compounds to calf-thymus DNA was studied by fluorescence spectroscopy, and docking simulations were used to predict the binding modes of these cyclic compounds. The spectral data demonstrated that all of these compounds can interact with CT-DNA. The sugar moiety played an important role in the process of binding between the intercalators containing glucuronic acid and DNA. The length and flexibility of the connecting bridges affected the binding affinity of the resultant cyclo-intercalators. Docking simulations showed that compounds 7 and 8 interact with DNA as mono-intercalators. |
| Databáze: | OpenAIRE |
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