Improved prediction of femoral fracture toughness in mice by combining standard medical imaging with Raman spectroscopy
| Autor: | Christine Massie, Andrew J. Berger, Hani A. Awad, Emma Knapp, Keren Chen |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
musculoskeletal diseases
Toughness Materials science 0206 medical engineering Osteoporosis Biomedical Engineering Biophysics 02 engineering and technology Spectrum Analysis Raman Article Mice 03 medical and health sciences symbols.namesake Absorptiometry Photon 0302 clinical medicine Fracture toughness Bone Density medicine Animals Humans Orthopedics and Sports Medicine Bone mineral Rehabilitation Fracture mechanics X-Ray Microtomography Bone fracture Femoral fracture medicine.disease 020601 biomedical engineering symbols Fracture (geology) Female Raman spectroscopy Femoral Fractures 030217 neurology & neurosurgery Biomedical engineering |
| Zdroj: | J Biomech |
| DOI: | 10.1101/2020.11.20.391383 |
| Popis: | Bone fragility and fracture risk are assessed by measuring the areal bone mineral density (aBMD) using dual-energy X-ray absorptiometry (DXA). While aBMD correlates with bone strength, it is a poor predictor of fragility fracture risk. Alternatively, fracture toughness assesses the bone’s resistance to crack propagation and fracture, making it a suitable bone quality metric. Here, we explored how femoral midshaft measurements from DXA, micro-computed tomography (μCT), and Raman spectroscopy could predict fracture toughness. We hypothesized that ovariectomy (OVX) decreases aBMD and fracture toughness compared to controls and we can optimize a multivariate assessment of bone quality by combining results from X-ray and Raman spectroscopy. Female mice underwent an OVX (n=5) or sham (n=5) surgery at 3 months of age. Femurs were excised 3 months after ovariectomy and assessed with Raman spectroscopy, μCT, and DXA. Subsequently, a notch was created on the anterior side of the mid-diaphysis of the femurs. Three-point bending induced a controlled fracture that initiated at the notch. The OVX mice had a significantly lower aBMD, cortical thickness, and fracture toughness when compared to controls (p2=0.71, p2=0.92, p |
| Databáze: | OpenAIRE |
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