High-speed interferometric imaging reveals dynamics of neuronal deformation during the action potential
| Autor: | Kevin C. Boyle, Valentina Zuckerman, Thomas Flores, Karl Deisseroth, Daniel Palanker, Tong Ling, Charu Ramakrishnan |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Physics Multidisciplinary Pixel Neurite Phase (waves) 02 engineering and technology Biological Sciences 021001 nanoscience & nanotechnology law.invention 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Confocal microscopy law Temporal resolution medicine Biophysics Neuron Sensitivity (control systems) 0210 nano-technology Optical path length |
| Zdroj: | Proc Natl Acad Sci U S A |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1920039117 |
| Popis: | Neurons undergo nanometer-scale deformations during action potentials, and the underlying mechanism has been actively debated for decades. Previous observations were limited to a single spot or the cell boundary, while movement across the entire neuron during the action potential remained unclear. Here we report full-field imaging of cellular deformations accompanying the action potential in mammalian neuron somas (−1.8 to 1.4 nm) and neurites (−0.7 to 0.9 nm), using high-speed quantitative phase imaging with a temporal resolution of 0.1 ms and an optical path length sensitivity of |
| Databáze: | OpenAIRE |
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