Estimation of the binding ability of main transport proteins of blood plasma with liver cirrhosis by the fluorescent probe method

Autor:	E. V. Korolik, E. A. Korolenko, V. V. Kirkovskii, A. K. Korolik
Rok vydání:	2007
Předmět:	chemistry.chemical_classification Quinaldine red biology Chemistry Serum albumin Albumin Nile red Plasma protein binding Condensed Matter Physics chemistry.chemical_compound Biochemistry Blood plasma biology.protein Glycoprotein Spectroscopy Whole blood
Zdroj:	Journal of Applied Spectroscopy. 74:561-566
ISSN:	1573-8647 0021-9037
Popis:	We present results from an investigation of the binding ability of the main transport proteins (albumin, lipoproteins, and α-1-acid glycoprotein) of blood plasma from patients at different stages of liver cirrhosis by the fluorescent probe method. We used the hydrophobic fluorescent probes anionic 8-anilinonaphthalene-1-sulfonate, which interacts in blood plasma mainly with albumin; cationic Quinaldine red, which interacts with α-1-acid glycoprotein; and neutral Nile red, which redistributes between lipoproteins and albumin in whole blood plasma. We show that the binding ability of albumin and α-1-acid glycoprotein to negatively charged and positively charged hydrophobic metabolites, respectively, increases in the compensation stage of liver cirrhosis. As the pathology process deepens and transitions into the decompensation stage, the transport abilities of albumin and α-1-acid glycoprotein decrease whereas the binding ability of lipoproteins remains high.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5bf15a252558624cab1531032249268a https://doi.org/10.1007/s10812-007-0088-6 Zobrazit plný text záznamu Plný text ve formátu PDF