Improved Protocols of ITS1-Based Metabarcoding and Their Application in the Analysis of Plant-Containing Products
Autor: | Anastasia A. Krinitsina, Viktoria Yu Shtratnikova, German A. Shipulin, Anna S. Speranskaya, E. V. Kupriyanova, D.O. Omelchenko, Anna Fedotova, Kamil Khafizov, Andrey A. Ayginin, Maria D. Logacheva, Denis V. Pozdyshev |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
tea lcsh:QH426-470 DNA Plant Library preparation ITS1 High resolution 01 natural sciences Article 03 medical and health sciences spice Genetics DNA Barcoding Taxonomic Food science Spices Genetics (clinical) Mathematics Repetitive Sequences Nucleic Acid business.industry 010401 analytical chemistry high-throughput sequencing Food safety DNA extraction Food Analysis 0104 chemical sciences lcsh:Genetics food safety 030104 developmental biology metabarcoding herbal medicine Negative correlation business |
Zdroj: | Genes Genes, Vol 10, Iss 2, p 122 (2019) Volume 10 Issue 2 |
ISSN: | 2073-4425 |
Popis: | Plants are widely used for food and beverage preparation, most often in the form of complex mixtures of dried and ground parts, such as teas, spices or herbal medicines. Quality control of such products is important due to the potential health risks from the presence of unlabelled components or absence of claimed ones. A promising approach to analyse such products is DNA metabarcoding due to its high resolution and sensitivity. However, this method’s application in food analysis requires several methodology optimizations in DNA extraction, amplification and library preparation. In this study, we present such optimizations. The most important methodological outcomes are the following: 1) the DNA extraction method greatly influences amplification success 2) the main problem for the application of metabarcoding is DNA purity, not integrity or quantity and 3) the “non-amplifiable” samples can be amplified with polymerases resistant to inhibitors. Using this optimized workflow, we analysed a broad set of plant products (teas, spices and herbal remedies) using two NGS platforms. The analysis revealed the problem of both the presence of extraneous components and the absence of labelled ones. Notably, for teas, no correlation was found between the price and either the absence of labelled components or presence of unlabelled ones for spices, a negative correlation was found between the price and presence of unlabelled components. |
Databáze: | OpenAIRE |
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