Prolonged zymosan-induced inflammatory pain hypersensitivity in mice lacking glycine receptor alpha2
| Autor: | Heinrich Betz, Ruirui Lu, Achim Schmidtko, Thomas Deller, Jessica Weiland, Wiebke Kallenborn-Gerhardt, Jana E. Lorenz, Gerd Geisslinger, Wei Gao, Bodo Laube, Domenico Del Turco |
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| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
In situ hybridization Mice Behavioral Neuroscience chemistry.chemical_compound Receptors Glycine Internal medicine medicine Animals Receptor Glycine receptor Inflammation Mice Knockout Neurons business.industry Zymosan Anatomy Spinal cord Endocrinology medicine.anatomical_structure Nociception Spinal Cord chemistry Hyperalgesia Synapses Knockout mouse Peripheral nerve injury business |
| Zdroj: | Behavioural Brain Research. 226:106-111 |
| ISSN: | 0166-4328 |
| Popis: | Glycinergic synapses play a major role in shaping the activity of spinal cord neurons under normal conditions and during persistent pain. However, the role of different glycine receptor (GlyR) subtypes in pain processing has only begun to be unraveled. Here, we analysed whether the GlyR alpha2 subunit might be involved in the processing of acute or persistent pain. Real-time RT-PCR and in situ hybridization analyses revealed that GlyR alpha2 mRNA is enriched in the dorsal horn of the mouse spinal cord. Mice lacking GlyR alpha2 (Glra2(-/-) mice) demonstrated a normal nociceptive behavior in models of acute pain and after peripheral nerve injury. However, mechanical hyperalgesia induced by peripheral injection of zymosan was significantly prolonged in Glra2(-/-) mice as compared to wild-type littermates. We conclude that spinal GlyRs containing the alpha2 subunit exert a previously unrecognized role in the resolution of inflammatory pain. |
| Databáze: | OpenAIRE |
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