Discrepancies in susceptibility test results for imipenem employing different in vitro test methods and DIN 58,940 breakpoints
| Autor: | H. Grimm, A. C. Rodloff, C. Hauss |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Microbiology (medical)
Imipenem In vitro test food.ingredient Colony Count Microbial Microbial Sensitivity Tests Biology Sensitivity and Specificity Agar dilution Microbiology Minimum inhibitory concentration food Reference Values Gram-Negative Bacteria medicine Agar Humans Food science Bacteriological Techniques Breakpoint Broth microdilution General Medicine Dilution Culture Media Infectious Diseases medicine.drug |
| Zdroj: | European journal of clinical microbiologyinfectious diseases : official publication of the European Society of Clinical Microbiology. 14(1) |
| ISSN: | 0934-9723 |
| Popis: | During the first half of 1993, bacteria that were isolated from clinical materials and found to have intermediate susceptibility by an agar dilution breakpoint method were collected in a large service laboratory in Germany. All of these isolates were gram-negative bacteria. They were re-tested employing full-scale agar dilution, broth microdilution, E-test and agar diffusion procedures. The results obtained indicated that 76.9% of the isolates were actually susceptible upon re-testing with a reference agar dilution technique. The reason for the discrepant results remained largely unclear. There was a high correlation between agar dilution and E-test results while the agreement with broth microdilution and agar diffusion was less satisfactory. It is suggested that the breakpoint between susceptible and intermediate categories currently recommended by DIN 58,940 (standard set by Deutsches Institut für Normung e.V.) be raised to reduce erroneous interpretations of minimum inhibitory concentrations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink