Substrate-induced chemiluminescence of xanthine oxidase and aldehyde oxidase
| Autor: | Richard M. Arneson |
|---|---|
| Rok vydání: | 1970 |
| Předmět: |
Niacinamide
Xanthine Oxidase Erythrocytes Chemical Phenomena Free Radicals Biophysics Photochemistry Biochemistry law.invention Superoxide dismutase chemistry.chemical_compound Sulfite law Animals Sulfites Cysteine Xanthine oxidase Hydrogen peroxide Molecular Biology Aldehyde oxidase Chemiluminescence Aldehydes biology Superoxide Nucleotides Hydrogen Peroxide Catalase Oxygen Chemistry Kinetics Milk chemistry Liver Luminescent Measurements biology.protein Cattle Rabbits Oxidoreductases |
| Zdroj: | Archives of biochemistry and biophysics. 136(2) |
| ISSN: | 0003-9861 |
| Popis: | Milk xanthine oxidase and rabbit liver aldehyde oxidase produce chemiluminescence when operating on their substrates. This luminescence can be measured by a Beckman scintillation counter with the coincidence circuit turned off. Hydrogen peroxide markedly enhances the luminescence, and catalase inhibits the light signal by more than 95%. The inhibition of luminescence by sulfite, cysteine, and bovine erythrocyte superoxide dismutase indicates that a free radical, probably the superoxide ion (O 2 ⨪), is responsible for the light signal. |
| Databáze: | OpenAIRE |
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