Lack of Protection in Mice and Necrotizing Bronchointerstitial Pneumonia with Bronchiolitis in Guinea Pigs Immunized with Vaccines Directed against the hsp60 Molecule ofMycobacterium tuberculosis
| Autor: | Oliver C. Turner, Alan D. Roberts, Anthony A. Frank, Susan W. Phalen, David M. McMurray, Jean Content, Olivier Denis, Sushila D'Souza, Audrey Tanghe, Kris Huygen, Ian M. Orme |
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| Rok vydání: | 2000 |
| Předmět: |
Tuberculosis
Guinea Pigs Immunology Microbiology Mycobacterium tuberculosis Mice Necrosis Plasmid Antigen Heat shock protein Pneumonia Bacterial Vaccines DNA medicine Animals Lung Tuberculosis Pulmonary biology Vaccination Chaperonin 60 medicine.disease biology.organism_classification Virology Infectious Diseases Microbial Immunity and Vaccines BCG Vaccine Bronchiolitis Parasitology HSP60 BCG vaccine |
| Zdroj: | Infection and Immunity. 68:3674-3679 |
| ISSN: | 1098-5522 0019-9567 |
| Popis: | In this study, the hsp60 and hsp70 heat shock protein antigens ofMycobacterium tuberculosiswere tested as potential vaccine candidates, using purified recombinant protein antigens or antigens encoded in the form of a DNA plasmid vaccine. Guinea pigs vaccinated with a mixture of the two proteins showed no evidence of resistance to low-dose aerosol challenge infection and quickly developed severe lung damage characterized by necrotizing bronchointerstitial pneumonia and bronchiolitis. As a result, we turned instead to a DNA vaccination approach using a plasmid encoding the hsp60 antigen ofM. tuberculosis. Although immunogenic in mice, vaccination with plasmid DNA encoding hsp60 was not protective in that model or in the guinea pig model and again gave rise to similar severe lung damage. This study seriously questions the safety of vaccines against tuberculosis that target highly conserved heat shock proteins. |
| Databáze: | OpenAIRE |
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