Mesoporous zirconia surfaces with anti-biofilm properties for dental implants
| Autor: | Roberto Chiesa, Alessandro Ettorre, Luigi De Nardo, Andrea Serafini, Alessandro C. Scalia, Stefania Cometa, Lia Rimondini, Agnese D’Agostino, Francesca Tana, Elvira De Giglio, Matteo Pavarini, Andrea Cochis |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Materials science
Surface Properties 0206 medical engineering Gingiva Biomedical Engineering chemistry.chemical_element Gallium Bioengineering 02 engineering and technology Osseointegration gallium (Ga) Biomaterials Coated Materials Biocompatible X-ray photoelectron spectroscopy Humans Cubic zirconia mesoporous structures zirconia (ZrO2 ) gallium (Ga) antibacterial dental coatings co-cultures cytocompatible Cells Cultured Dental Implants mesoporous structures Osteoblasts Bacteria co-cultures antibacterial dental coatings Fibroblasts 021001 nanoscience & nanotechnology Microstructure 020601 biomedical engineering Coculture Techniques Anti-Bacterial Agents zirconia (ZrO2 ) Chemical engineering chemistry Transmission electron microscopy Biofilms Zirconium cytocompatible Selected area diffraction 0210 nano-technology Mesoporous material |
| Zdroj: | Biomedical Materials. 16:045016 |
| ISSN: | 1748-605X 1748-6041 |
| Popis: | Cytocompatible bioactive surface treatments conferring antibacterial properties to osseointegrated dental implants are highly requested to prevent bacteria-related peri-implantitis. Here we focus on a newly designed family of mesoporous coatings based on zirconia (ZrO2) microstructure doped with gallium (Ga), exploiting its antibacterial and pro-osseo-integrative properties. The ZrO2 films were obtained via sol–gel synthesis route using Pluronic F127 as templating agent, while Ga doping was gained by introducing gallium nitrate hydrate. Chemical characterization by means of x-ray photoelectron spectroscopy and glow discharge optical emission spectroscopy confirmed the effective incorporation of Ga. Then, coatings morphological and structural analysis were carried out by transmission electron microscopy and selected area electron diffraction unveiling an effective stabilization of both the mesoporous structure and the tetragonal ZrO2 phase. Specimens’ cytocompatibility was confirmed towards gingival fibroblast and osteoblasts progenitors cultivated directly onto the coatings showing comparable metabolic activity and morphology in respect to controls cultivated on polystyrene. The presence of Ga significantly reduced the metabolic activity of the adhered oral pathogens Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans in comparison to untreated bulk zirconia (p < 0.05); on the opposite, Ga ions did not significantly reduce the metabolism of the oral commensal Streptococcus salivarius (p > 0.05) thus suggesting for a selective anti-pathogens activity. Finally, the coatings’ ability to preserve cells from bacterial infection was proved in a co-culture method where cells and bacteria were cultivated in the same environment: the presence of Ga determined a significant reduction of the bacteria viability while allowing at the same time for cells proliferation. In conclusion, the here developed coatings not only demonstrated to satisfy the requested antibacterial and cytocompatibility properties, but also being promising candidates for the improvement of implantable devices in the field of implant dentistry. |
| Databáze: | OpenAIRE |
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