NO involvement in the inhibition of ghrelin on voltage-dependent potassium currents in rat hippocampal cells
| Autor: | Hongli Sun, Yong Lu, Qian Su, Tianwei Lin, Zhongliang Zhu, Junli Zhang, Yurong Zhang, Xu Wang, Shaokang Dang, Hui Li, Lin Zhang, Xiaoxiao Zhang, Huiping Zhang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Patch-Clamp Techniques Potassium Channels Arginine media_common.quotation_subject Potassium chemistry.chemical_element Hippocampal formation Peptide hormone Nitric Oxide Hippocampus Nitric oxide Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Animals Molecular Biology Inhibitory effect media_common Chemistry General Neuroscience digestive oral and skin physiology Appetite Ghrelin Rats NG-Nitroarginine Methyl Ester 030104 developmental biology Endocrinology Potassium Channels Voltage-Gated Neurology (clinical) hormones hormone substitutes and hormone antagonists 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Brain Research. 1678:40-46 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/j.brainres.2017.09.031 |
| Popis: | Ghrelin is a peptide hormone that plays an important role in promoting appetite, regulating distribution and rate of use of energy, cognition, and mood disorders, but the relevant neural mechanisms of these function are still not clear. In this study, we examined the effect of ghrelin on voltage-dependent potassium (K+) currents in hippocampal cells of 1–3 days SD rats by whole-cell patch-clamp technique, and discussed whether NO was involved in this process. The results showed that ghrelin significantly inhibited the voltage-dependent K+ currents in hippocampal cells, and the inhibitory effect was more significant when l -arginine was co-administered. In contrast, N-nitro- l -arginine methyl ester increased the ghrelin inhibited K+ currents and attenuated the inhibitory effect of ghrelin. While d -arginine (D-AA) showed no significant impact on the ghrelin-induced decrease in K+ current. These results show that ghrelin may play a physiological role by inhibiting hippocampal voltage dependent K+ currents, and the NO pathway may be involved in this process. |
| Databáze: | OpenAIRE |
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