Polar mixture analysis by NMR under spin diffusion conditions in viscous sucrose solution and agarose gel
| Autor: | François Pedinielli, Simon Mougeolle, Jean-Marc Nuzillard, Pedro Lameiras |
|---|---|
| Přispěvatelé: | Chimie des Substances Naturelles (ICMRCSN), Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2019 |
| Předmět: |
[CHIM.ORGA]Chemical Sciences/Organic chemistry
Chemistry Chemical polarity Analytical chemistry 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Homonuclear molecule 0104 chemical sciences NMR spectra database chemistry.chemical_compound Heteronuclear molecule [CHIM.ANAL]Chemical Sciences/Analytical chemistry Spin diffusion Molecule Agarose Condensed Matter::Strongly Correlated Electrons Physical and Theoretical Chemistry 0210 nano-technology Two-dimensional nuclear magnetic resonance spectroscopy |
| Zdroj: | Faraday Discussions Faraday Discussions, Royal Society of Chemistry, 2019, 218, pp.233-246. ⟨10.1039/C8FD00226F⟩ |
| ISSN: | 1364-5498 1359-6640 |
| DOI: | 10.1039/C8FD00226F⟩ |
| Popis: | International audience; The use of two new viscous solvents, sucrose solution and agarose gel, is reported for the first time for giving access to the individual NMR spectra of polar and potentially bioactive compounds in a mixture. Under viscous conditions, the tumbling rate of small and mid-sized molecules reduces in solution, so that the longitudinal cross-relaxation encourages the observation of spin diffusion. As a result, all of the resonances of the 1H nuclei within the same molecule tend to correlate together in a 2D NOESY spectrum, thus paving the way to mixture analysis. This work describes the individualization of four structurally close mixed dipeptides: Leu–Val, Leu–Tyr, Gly–Tyr and Ala–Tyr dissolved in each of sucrose solution and agarose gel, by means of spin diffusion in homonuclear selective 1D NOESY, selective 2D NOESY experiments and heteronuclear 2D HSQC-NOESY. Sucrose solution should be preferred to agarose gel for the investigation of mixtures made of small and flexible polar compounds, due to its capability to give rise to more suitable viscous conditions mandatory for efficient spin diffusion, even though agarose gel reveals the benefit of not offering intense residual solvent proton signals due to active transverse relaxation. |
| Databáze: | OpenAIRE |
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