The Effect of a Fast-Releasing Hydrogen Sulfide Donor on Vascularization of Subcutaneous Scaffolds in Immunocompetent and Immunocompromised Mice

Autor: Michael Alexander, Elliot L. Botvinick, Jonathan R. T. Lakey, Avid Najdahmadi, Paul de Vos, Samuel Rodriquez, Shiri Li, Jan-Luuk Hillebrands, Alexandra M. Smink, Harry van Goor
Přispěvatelé: Groningen Institute for Organ Transplantation (GIOT), Groningen Kidney Center (GKC), Man, Biomaterials and Microbes (MBM), Translational Immunology Groningen (TRIGR)
Rok vydání: 2020
Předmět:
0301 basic medicine
CD31
Angiogenesis
Nude
lcsh:QR1-502
Islets of Langerhans Transplantation
hydrogen sulfide
030204 cardiovascular system & hematology
Inbred C57BL
Biochemistry
lcsh:Microbiology
chemistry.chemical_compound
Mice
0302 clinical medicine
Gene expression
geography.geographical_feature_category
Diabetes
Endoglin
Islet
Platelet Endothelial Cell Adhesion Molecule-1
Injections
Intraperitoneal
medicine.medical_specialty
Mice
Nude
Neovascularization
Physiologic
Sodium hydrosulfide
Article
Injections
03 medical and health sciences
Immunocompromised Host
Islets of Langerhans
Immune system
vascularization
Internal medicine
medicine
Animals
Intraperitoneal
Physiologic
Molecular Biology
Neovascularization
Transplantation
geography
subcutaneous scaffolds
Mice
Inbred C57BL
immune system
030104 developmental biology
Endocrinology
chemistry
Biochemistry and Cell Biology
Zdroj: Biomolecules
Biomolecules, 10(5):722. MDPI AG
Volume 10
Issue 5
Biomolecules, Vol 10, Iss 722, p 722 (2020)
Biomolecules, vol 10, iss 5
ISSN: 2218-273X
Popis: Islet transplantation into subcutaneous polymer scaffolds has shown to successfully induce normoglycemia in type 1 diabetes models. Vascularization of these scaffolds is imperative for optimal control of glucose levels. We studied the effect of the vascular stimulator hydrogen sulfide (H2S) on the degree of vascularization of a scaffold and the role of the immune system in this process. Scaffolds were subcutaneously implanted in immunocompetent C57BL/6 and immunocompromised nude mice. Mice received twice-daily intraperitoneal injections of the fast-releasing H2S donor sodium hydrosulfide (NaHS, 25 or 50 &mu
mol/kg) or saline for 28 days. After 63 days the vascular network was analyzed by histology and gene expression. Here we showed that the vascularization of a subcutaneous scaffold in nude mice was significantly impaired by H2S treatment. Both the CD31 gene and protein expression were reduced in these scaffolds compared to the saline-treated controls. In C57BL/6 mice, the opposite was found, the vascularization of the scaffold was significantly increased by H2S. The mRNA expression of the angiogenesis marker CD105 was significantly increased compared to the controls as well as the number of CD31 positive blood vessels. In conclusion, the immune system plays an important role in the H2S mediated effect on vascularization of subcutaneous scaffolds.
Databáze: OpenAIRE
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