Characteristics of a foot-and-mouth disease virus with a partial VP1 G-H loop deletion in experimentally infected cattle
| Autor: | Veronica L. Fowler, Satya Parida, Anette Bøtner, Paul V. Barnett, John B. Bashiruddin, Nick J. Knowles, Graham J. Belsham, B. Bankowski, Carolina Stenfeldt |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
viruses Cattle Diseases Virulence Biology Antibodies Viral Microbiology Virus Serology Tongue medicine Animals Amino Acid Sequence Sequence Deletion General Veterinary Foot-and-mouth disease Vaccination General Medicine biology.organism_classification medicine.disease Virology Foot-and-Mouth Disease Virus Foot-and-Mouth Disease biology.protein Capsid Proteins Cattle Foot-and-mouth disease virus Antibody |
| Zdroj: | Veterinary Microbiology. 169:58-66 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/j.vetmic.2013.12.008 |
| Popis: | Previous work in cattle illustrated the protective efficacy and negative marker potential of a A serotype foot-and-mouth disease virus (FMDV) vaccine prepared from a virus lacking a significant portion of the VP1 G-H loop (termed A(-)). Since this deletion also includes the arginine-glycine-aspartate (RGD) motif required for virus attachment to the host cell in vivo, it was hypothesised that this virus would be attentuated in naturally susceptible animals. The A(-) virus was passaged three times in cattle via needle inoculation of virus suspension delivered into the intradermal space of the tongue (intradermolingual: IDL). Included in the study were three direct contact cattle, two of which were used for the third cattle passage (by inoculation) after direct contact exposure for three days. Cattle were monitored for clinical signs and samples were collected for sequencing as well as antibody and viral genome detection by ELISA and qRT-PCR. Following needle inoculation with the A(-) virus, naïve cattle developed typical clinical signs of FMDV infection, diagnostic assays also provided positive serological and virological results. However, the contact cattle did not develop clinical signs or generate serological or virological markers indicative of FMDV infection even when the cattle were subsequently needle inoculated with 10(5) TCID50 A(-) FMDV delivered IDL following three days of direct contact exposure. The results suggest that the A(-) virus is not attentuated in cattle when inoculated IDL. This virus could be useful as a tool to understand further the natural pathogenesis, receptor usage and internalisation pathways of FMDV. |
| Databáze: | OpenAIRE |
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