| Autor: |
Houtman, Evelyn, Tuerlings, Margo, Riechelman, Janne, Suchiman, Eka H. E. D., van der Wal, Robert J. P., Nelissen, Rob G. H. H., Mei, Hailiang, Ramos, Yolande F. M., Coutinho de Almeida, Rodrigo, Meulenbelt, Ingrid |
| Rok vydání: |
2021 |
| DOI: |
10.6084/m9.figshare.15179504 |
| Popis: |
Additional file 1. Supplementary Tables and Figures. List of content: Supplementary Table S1. [a] Donor characteristics of samples for which RNA was sequenced. [b] Donor characteristics of independent samples used for replication of RNA-sequencing findings. Supplementary Table S2. Primer sequences used for replication and validation by RT-qPCR. Supplementary Table S3. Genes differentially expressed in 65%MS (DEMS) cartilage compared to control cartilage of human osteochondral explants. Supplementary Table S4. Gene enrichment found in Enrichr. Enrichment for [a] 156 DEMS genes in and [b] 92 DEExclusiveMS for the gene ontology terms: biological process, molecular function and cellular component 2018, and pathways: KEGG 2019 human and reactome. Supplementary Table S5. DEMS genes coinciding with previously reported DE genes in OA pathophysiology (DEOA). [a] DEMS genes with same direction of effect as DEOA genes. [b] DEMS genes with opposite direction of effect as DEOA genes. Supplementary Table S6. Exclusive mechanical response genes (DEExclusiveMS). Supplementary Table S7. Previously reported OA risk loci present in our DE gene dataset. Supplementary Table S8. All insulin growth factor binding proteins (IGFBPs) and related DE genes identified in our analysis. Supplementary Figure S1. Venn diagram of coinciding genes between differentially expressed genes in mechanically stressed versus control cartilage from osteochondral explants (DEMS) and previously identified differentially expressed genes in preserved versus lesioned OA cartilage (DEOA). Supplementary Figure S2. Protein-protein interaction network in STRING of proteins encoded by differentially expressed genes (N = 92 genes) not coinciding with OA pathophysiology (DEExclusiveMS). Supplementary Figure S3. Heat-map of proteins present in SASP. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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