Detection by radioimmunoassay of antibodies in human smallpox patients and vaccinees
Autor: | J P Koplan, J H Nakano, D W Ziegler, H D Hutchinson |
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Rok vydání: | 1975 |
Předmět: |
Microbiology (medical)
Hemagglutination Inhibition Tests Hemagglutination viruses Radioimmunoassay Vaccinia virus Biology Antibodies Viral chemistry.chemical_compound Plaque reduction neutralization test Neutralization Tests Humans Hemagglutination assay Complement Fixation Tests Vaccination Antibody titer Hemagglutination Tests Variola virus Complement fixation test Virology chemistry Evaluation Studies as Topic Antibody Formation Immunology Vaccinia Research Article Smallpox |
Zdroj: | Journal of Clinical Microbiology. 1:311-317 |
ISSN: | 1098-660X 0095-1137 |
DOI: | 10.1128/jcm.1.3.311-317.1975 |
Popis: | A radioimmunoassay procedure was developed for determining smallpox and vaccinia antibodies in human sera. The test detected and measured both primary and secondary immune responses in persons infected with variola virus or vaccinia virus. The antibody titers obtained by complement fixation, hemagglutination inhibition, plaque reduction neutralization, and radioimmunoassay methods were compared. In sequential serum specimens, the radioimmunoassay test indicated fourfold or greater increases in all of the smallpox patients and in six of eight vaccinated persons. Both the complement fixation and the hemagglutination inhibition tests were less effective. In persons who had been vaccinated, radioimmunoassay and plaque reduction neutralization tests appeared to measure the same immune response. However, in smallpox patients the immune response was readily detected by radioimmunoassay, whereas an immune response was not detected by the plaque reduction neutralization test when vaccinia virus was the antigen in the test system. Radioimmunoassay is an operationally simple procedure which provides objective and quantitative end-point titers in serological determinations. |
Databáze: | OpenAIRE |
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