High-density lipoproteins downregulate CCL2 production in human fibroblast-like synoviocytes stimulated by urate crystals
| Autor: | Leonardo Punzi, Anna Scanu, Carlo Agostini, Danielle Burger, Federica Frezzato, Assunta Pozzuoli, Lyssia Gruaz, Paolo Sfriso, Francesca Oliviero |
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| Rok vydání: | 2010 |
| Předmět: |
Uric Acid/adverse effects/*metabolism
Gout Immunology Down-Regulation Enzyme-Linked Immunosorbent Assay CCL2 Biology Gout/metabolism chemistry.chemical_compound Lipoproteins HDL/*metabolism Synovial Membrane/cytology/drug effects/*metabolism Rheumatology Downregulation and upregulation Research article Fibroblasts/drug effects/*metabolism medicine Humans Immunology and Allergy Fibroblast Cells Cultured Chemokine CCL2 Chemokine CCL2/*biosynthesis ddc:616 Microscopy Confocal Monocyte Synovial Membrane Chemotaxis Fibroblasts medicine.disease Uric Acid Cell biology medicine.anatomical_structure chemistry Biochemistry Uric acid Synovial membrane Crystallization Lipoproteins HDL |
| Zdroj: | Arthritis Research & Therapy Arthritis Research and Therapy, Vol. 12, No 1 (2010) P. R23 |
| ISSN: | 1478-6354 |
| DOI: | 10.1186/ar2930 |
| Popis: | INTRODUCTION: To investigate whether monosodium urate (MSU) crystals induce the production of CCL2 (monocyte chemoattractant protein-1; MCP-1) in human fibroblast-like synoviocytes (FLS) and whether this mechanism would be affected by high-density lipoproteins (HDL). METHODS: Human FLS isolated from synovial tissue explants were stimulated with MSU crystals (0.01 to 0.5 mg/ml) or interleukin (IL)-1beta (10 pg/ml) in the presence or absence of HDL (50 and 100 microg/ml). The production and expression of CCL2 was evaluated with ELISA, confocal microscopy, immunofluorescence microscopy, chemotaxis assay, and real-time quantitative PCR. RESULTS: Exposure of FLS to MSU crystals induced CCL2 accumulation in culture medium in a dose- and time-dependent manner, reaching a plateau at 50 to 75 microg/ml MSU crystals and 20 to 24 hours. Although low, the induced CCL2 levels were sufficient to trigger mononuclear cell migration. In resting FLS, CCL2 was localized in small cytoplasmic vesicles whose number diminished with MSU crystal stimulation. Concomitantly, MSU crystals triggered the induction of CCL2 mRNA expression. All these processes were inhibited by HDL, which cause a 50% decrease in CCL2 mRNA levels and a dose-dependent inhibition of the release of CCL2. Similar results were obtained when FLS were pretreated with HDL and washed before activation by MSU crystals or IL-1beta, suggesting a direct effect of HDL on the FLS activation state. CONCLUSIONS: The present results demonstrate that MSU crystals induce FLS to release CCL2 that is stored in vesicles in resting conditions. This mechanism is inhibited by HDL, which may limit the inflammatory process by diminishing CCL2 production and, in turn, monocytes/macrophages recruitment in joints. This study confirms the antiinflammatory functions of HDL, which might play a part in the limitation of acute gout attack. |
| Databáze: | OpenAIRE |
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