Regulation of neural stem cells by choroid plexus cells population
| Autor: | Aline Fernanda de Souza, Natalia Juliana Nardelli Gonçalves, Naira Caroline Godoy Pieri, Kelly Cristine Santos Roballo, André Furugen Cesar de Andrade, Carlos Eduardo Ambrósio |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Swine Biology Nestin 03 medical and health sciences 0302 clinical medicine Cerebrospinal fluid Neural Stem Cells Neurosphere Glial Fibrillary Acidic Protein Telocyte medicine Animals CD90 Progenitor cell Induced pluripotent stem cell 5'-Nucleotidase Cells Cultured General Neuroscience Neural stem cell 030104 developmental biology Choroid Plexus Leukocyte Common Antigens Thy-1 Antigens Choroid plexus Neuroglia Octamer Transcription Factor-3 030217 neurology & neurosurgery |
| Zdroj: | Neuroscience Letters. 626:35-41 |
| ISSN: | 0304-3940 |
| Popis: | The choroid plexus is a tissue on the central nervous system responsible for producing cerebrospinal fluid, maintaining homeostasis and neural stem cells support; though, all of its functions still unclear. This study aimed to demonstrate the niches of choroid plexus cells for a better understanding of the cell types and functions, using the porcine as the animal model. The collected material was analyzed by histology, immunohistochemistry, and cell culture. The cell culture was characterizated by immunocytochemistry and flow cytometry. Our results showed OCT-4, TUBIII, Nestin, CD45, CD73, CD90 positive expression and GFAP, CD105 negative expression, also methylene blue histological staining confirmed the presence of telocytes cells. We realized that the choroid plexus is a unique and incomparable tissue with different niches of cells as pluripotent, hematopoietic, neuronal progenitors and telocyte cells, which provide its complexity, differentiated functionality and responsibility on brain balance and neural stem cells regulation. |
| Databáze: | OpenAIRE |
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