Embryotoxicity of magainin-2-amide and its enhancement by cyclodextrin, albumin, hydrogen peroxide and acidification
| Autor: | Ewa T. Mystkowska, Aldona Komar, Wojciech Sawicki, Anna Niemierko |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
Embryonic Development Xenopus Proteins Magainins chemistry.chemical_compound Mice Anti-Infective Agents Pregnancy Culture Techniques Animals Propidium iodide Viability assay Bovine serum albumin Hydrogen peroxide chemistry.chemical_classification Cyclodextrins biology Cyclodextrin Dose-Response Relationship Drug Rehabilitation Albumin Magainin Obstetrics and Gynecology Drug Synergism Serum Albumin Bovine Hydrogen Peroxide Hydrogen-Ion Concentration Embryo Mammalian In vitro Kinetics Reproductive Medicine chemistry Biochemistry biology.protein Female Antimicrobial Cationic Peptides |
| Zdroj: | Human reproduction (Oxford, England). 16(7) |
| ISSN: | 0268-1161 |
| Popis: | BACKGROUND The channel-forming antimicrobial peptide, magainin-2-amide, interacts preferentially with negatively charged, non cholesterol-containing membranes, including those of sperm, oocytes and cells of pre-implantation embryos. Cyclodextrin and albumin remove membrane cholesterol and together with hydrogen peroxide (H2O2) are potential enhancers of embryotoxicity. METHODS Two-cell murine embryos were cultured in vitro with magainin-2-amide at a high effective concentration (250 microg/ml) and at subthreshold concentrations (166 and 200 microg/ml). Embryos treated with sub-threshold concentrations of magainin were additionally treated with cyclodextrin, bovine serum albumin or H2O2 or were cultured under acidified conditions. Cell viability was verified with propidium iodide and fluorescein diacetate. RESULTS The embryotoxic effect of magainin and H2O2 was dose- and time-dependent. Cyclodextrin, H2O2, acidification of the medium, and to a lesser extent albumin, enhanced the embryotoxicity of magainin at sub-threshold concentrations. CONCLUSION Magainin on its own is highly embryotoxic. Its embryotoxicity is enhanced by cyclodextrin, albumin, H2O2 and acidification. Thus, magainin which has antibacterial, antifungal and antiprotozoal activity may also have a potential role as a contraceptive agent. The harmful effects of various concentrations of the exogenous H2O2 on 2-cell stage mouse embryos are reported here, to the best of our knowledge, for the first time. |
| Databáze: | OpenAIRE |
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