Molecular Mechanisms That Contribute to Horizontal Transfer of Plasmids by the Bacteriophage SPP1
| Autor: | Alexei Sorokin, Silvia Ayora, Ana Valero-Rello, Alvaro Quevedo-Olmos, María López-Sanz |
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| Přispěvatelé: | Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris Saclay (COmUE), CSIC, Ctr Nacl Biotecnol, Dept Microbial Biotechnol, Madrid, Spain, Partenaires INRAE, Spanish from MINECO [BFU201239879-C02-02, BFU2015-67065-P], PathoBactEvol from ANR [ANR-12-ADAP-0018], Valero Rello, Ana, López-Sanz, María, Ayora Hirsch, Sylvia |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) bacteriophages antibiotic resistance [SDV]Life Sciences [q-bio] lcsh:QR1-502 Biology Microbiology lcsh:Microbiology horizontal gene transfer plasmid transduction SPP1 Bacteriophage 03 medical and health sciences chemistry.chemical_compound Transduction (genetics) Plasmid Original Research Plasmid preparation DNA replication biology.organism_classification Molecular biology 030104 developmental biology chemistry Lytic cycle Replisome DNA |
| Zdroj: | Frontiers in Microbiology Frontiers in Microbiology, Vol 8 (2017) Frontiers in Microbiology, Frontiers Media, 2017, 8, ⟨10.3389/fmicb.2017.01816⟩ Frontiers in Microbiology (8), . (2017) |
| ISSN: | 1664-302X |
| DOI: | 10.3389/fmicb.2017.01816⟩ |
| Popis: | International audience; Natural transformation and viral-mediated transduction are the main avenues of horizontal gene transfer in Firmicutes. Bacillus subtilis SPP1 is a generalized transducing bacteriophage. Using this lytic phage as a model, we have analyzed how viral replication and recombination systems contribute to the transfer of plasmid-borne antibiotic resistances. Phage SPP1 DNA replication relies on essential phage-encoded replisome organizer (G38P), helicase loader (G39P), hexameric replicative helicase (G40P), recombinase (G35P) and in less extent on the partially dispensable 5 0 ! 3 0 exonuclease (G34.1P), the single-stranded DNA binding protein (G36P) and the Holliday junction resolvase (G44P). Correspondingly, the accumulation of linear concatemeric plasmid DNA, and the formation of transducing particles were blocked in the absence of G35P, G38P, G39P, and G40P, greatly reduced in the G34.1P, G36P mutants, and slightly reduced in G44P mutants. In contrast, establishment of injected linear plasmid DNA in the recipient host was independent of viral-encoded functions. DNA homology between SPP1 and the plasmid, rather than a viral packaging signal, enhanced the accumulation of packagable plasmid DNA. The transfer efficiency was also dependent on plasmid copy number, and rolling-circle plasmids were encapsidated at higher frequencies than theta-type replicating plasmids. |
| Databáze: | OpenAIRE |
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