Ets-1 Activates the DRA Promoter in B Cells
| Autor: | B M Peterlin, Nabila Jabrane-Ferrat |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Pyrimidine
Molecular Sequence Data Regulatory Factor-X HLA-DR alpha-Chains Regulatory Factor X Transcription Factors Biology Major histocompatibility complex Cell Line Proto-Oncogene Protein c-ets-1 chemistry.chemical_compound Proto-Oncogene Proteins Humans Point Mutation Binding site Promoter Regions Genetic Gene Molecular Biology B-Lymphocytes Binding Sites Base Sequence Proto-Oncogene Proteins c-ets Promoter DNA HLA-DR Antigens Cell Biology Molecular biology DNA-Binding Proteins Gene Expression Regulation chemistry biology.protein Research Article Protein Binding Transcription Factors |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1098-5549 |
| DOI: | 10.1128/mcb.14.11.7314-7321.1994 |
| Popis: | The X box in promoters of class II major histocompatibility complex genes plays a crucial role in the B-cell-specific and gamma interferon-inducible expression of these genes. The sequence TTCC is located in the pyrimidine tract which extends 5' to and partially overlaps the X box of the DRA promoter. This sequence resembles the core binding site for the Ets family of DNA-binding proteins. In this study, we demonstrate that mutations within the pyrimidine tract which change the TTCC motif, but do not affect the binding of regulatory factor X to the X box, decrease the activity of the DRA promoter in B cells. Furthermore, using electrophoretic mobility shift assays and cotransfection experiments, we demonstrate that Ets-1, but not Ets-2 or PU.1, functionally interacts with the pyrimidine tract and activates the DRA promoter. |
| Databáze: | OpenAIRE |
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