Proteome Analysis of Bronchoalveolar Lavage Fluid in Lung Fibrosis Associated with Systemic Sclerosis
| Autor: | Tsukasa Okamoto, Yasunari Miyazaki, Naohiko Inase, Ryutaro Shirahama, Yasuyuki Yoshizawa |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
lcsh:Immunologic diseases. Allergy
Male Pathology medicine.medical_specialty Proteome Pulmonary Fibrosis Inflammation Scleroderma Nephrogenic Fibrosing Dermopathy Pathogenesis Heat shock protein Pulmonary fibrosis medicine bronchoalveolar lavage Humans Immunology and Allergy scleroderma alpha-Macroglobulins skin and connective tissue diseases Heat-Shock Proteins Aged Glutathione Transferase Pulmonary Surfactant-Associated Protein A integumentary system medicine.diagnostic_test business.industry Interstitial lung disease General Medicine Middle Aged medicine.disease Toll-like receptors Bronchoalveolar lavage inflammation Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization alpha 1-Antitrypsin Immunology Female medicine.symptom lcsh:RC581-607 business Bronchoalveolar Lavage Fluid |
| Zdroj: | Allergology International, Vol 59, Iss 4, Pp 409-415 (2010) |
| ISSN: | 1323-8930 |
| Popis: | Background Interstitial lung disease (ILD) is the major cause of mortality in collagen vascular diseases. However, its pathogenesis still needs to be elucidated. Methods To evaluate the alteration of certain proteins in bronchoalveolar lavage fluid (BALF) and clarify the causative role in the processes of ILD in systemic sclerosis (SSc), we compared a BALF protein profile between 5 patients with systemic sclerosis with pulmonary fibrosis (SSc-fib +) and 4 patients with systemic sclerosis without pulmonary fibrosis (SSc-fib-) using two-dimensional gel electrophoresis (2-DE), and matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS). Results We analyzed spots over the range of 10.1 kDa to 207.4 kDa. SSc-fib + patients showed increased 3 proteins compared to SSc-fib- including α2-macroglobulin, α1-antitrypsin, and pulmonary surfactant protein A and decreased 2 proteins including α2 heat shock protein (HSP) and glutathione S-transferase (GST) compared to SSc-fib- patients. Conclusions In conclusion, we identified several interesting proteins that might have roles in ILD of SSc patients. Further studies are warranted to clarify the role of these proteins in the processes of pulmonary fibrosis in SSc. |
| Databáze: | OpenAIRE |
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