Microwave superheating enhances immunocyto-chemistry in the freshly frozen rat brain
| Autor: | Robert K. Kan, Jonathan R. Day, Barbara W. DeHart |
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| Rok vydání: | 1996 |
| Předmět: |
Male
Pathology medicine.medical_specialty Central nervous system Immunocytochemistry Biology Hippocampus Stain chemistry.chemical_compound Antigen medicine Animals Microwaves Cerebral Cortex General Neuroscience Brain Immunohistochemistry Rats Inbred F344 Rats Staining medicine.anatomical_structure Antigen retrieval chemistry biology.protein Antibody Microwave |
| Zdroj: | NeuroReport. 7:2691-2694 |
| ISSN: | 0959-4965 |
| DOI: | 10.1097/00001756-199611040-00057 |
| Popis: | The goal of this study was to evaluate the effects of microwave irradiation on immunocytochemical staining in freshly frozen rat brain tissue using an antibody previously shown to successfully stain only formalin-fixed materials. GFAP immunoreactivity was markedly increased in sections exposed to microwave heating when compared with control tissue. Uniform staining enhancement was mainly a result of increased numbers of immunoreactive soma and large astrocytic processes. The integrity of the tissue was not affected by this treatment. Other antibodies need to be tested with this method, but our results suggest that microwave irradiation is useful for rapid antigen retrieval in freshly frozen central nervous system tissue. |
| Databáze: | OpenAIRE |
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