Effects of the application of the amniotic membrane in the healing process of skin wounds in rats
| Autor: | Daniel Cabral Leão Ferreira, Rauirys Alencar de Oliveira, Mariana Barbosa Dias Campelo, Emília Ângela Loschiavo Arisawa, Leonardo Fonseca Maia, Antônio Luiz Martins Maia Filho, Luciana Barros Sant'Anna, J. F. Santos |
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| Rok vydání: | 2017 |
| Předmět: |
Male
RD1-811 Skin wound Inflammation Collagen Type I Andrology 030207 dermatology & venereal diseases 03 medical and health sciences Random Allocation 0302 clinical medicine Medicine Animals Humans Amnion Rats Wistar Fibroblast Skin Wound Healing Biological Dressings business.industry Rats Fibroblasts Surgical Injury Disease Models Animal Membrane medicine.anatomical_structure Collagen Type III 030220 oncology & carcinogenesis Surgery Collagen medicine.symptom business Wound healing Type I collagen |
| Zdroj: | Acta Cirurgica Brasileira, Volume: 33, Issue: 2, Pages: 144-155, Published: FEB 2018 Acta Cirúrgica Brasileira v.33 n.2 2018 Acta Cirúrgica Brasileira Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC Acta Cirurgica Brasileira, Vol 33, Iss 2, Pp 144-155 (2018) |
| ISSN: | 1678-2674 |
| Popis: | Purpose: To evaluate the efficacy of the application of the human amniotic membrane (HAM) on the inflammatory process, fibroblast proliferation, formation of collagenand reduction of skin wound areas in rats. Methods: Thirty six rats were submitted to a surgical injury induction and divided into two groups (n = 18): group C (control) and T (treated with the HAM). The macroscopic evolution in the wound area and the histological characteristics of the skin samples were evaluated. Results: The regression of the wound area was greater in group T. The histological analysis revealed a significant reduction (p < 0.05) in the inflammatory infiltrate in group T at all experimental periods compared with that in the control group. Furthermore, the group T presented a significant increase in the proliferation of fibroblasts at 14 and 21 days compared with group C (p < 0.05). Regarding the deposition of mature collagen fibers, there was an increase in the replacement of type III collagen by type I collagen in group T (p < 0.05). Conclusion: Treatment with the HAM reduced the healing time as well as the inflammatory responses, increased the proliferation of fibroblasts, and induced a higher concentration of mature collagen fibers. |
| Databáze: | OpenAIRE |
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