Relationship Between Heat Shock Protein Induction and the Binding of Antibodies to the Extractable Nuclear Antigens on Cultured Human Keratinocytes
| Autor: | Fukumi Furukawa, Norihisa Matsuyoshi, Kyoko Shimizu, Kouichi Ikai, Sadao Imamura |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Keratinocytes
Male Anti-nuclear antibody Extractable nuclear antigens medicine.drug_class Cell Survival Cell Separation Dermatology Biology Monoclonal antibody Immunofluorescence Biochemistry Antigen Heat shock protein medicine Ultraviolet light Humans Molecular Biology Cells Cultured Heat-Shock Proteins Autoantibodies Lupus erythematosus medicine.diagnostic_test Prostaglandin D2 Infant Newborn Nuclear Proteins Antigens Nuclear Cell Biology medicine.disease Flow Cytometry Molecular biology Immunoglobulin G Immunology Binding Sites Antibody |
| Zdroj: | Journal of Investigative Dermatology. 101(2):191-195 |
| ISSN: | 0022-202X |
| DOI: | 10.1111/1523-1747.ep12363785 |
| Popis: | The importance of environmental factors such as ultraviolet light and temperature in the pathogenesis of cutaneous lupus erythematosus is well recognized. Recent evidence suggests the presence of autoantibodies to heat shock proteins (HSP) in the sera and enhanced expression of the HSP70 gene in peripheral blood mononuclear cells of patients with systemic lupus erythematosus. We designed experiments to determine how HSP or stress protein inducers affect the cell surface binding of IgG antibodies from sera containing anti-SS-A/ Ro and anti-ribonuclear protein (RNP) antibodies to keratinocytes because these antibodies are considered to be one of the immunologic triggers of cutaneous lupus erythematosus. Immunofluorescence and immunoblot analysis using a monoclonal antibody to the 72 kDa of HSP revealed that an 18-h incubation with 10 μg/ml of Δ 12 - PGJ 2 , one of cytotoxic prostaglandins, induced HSP72 formation in cultured human keratinocytes. Δ 12 -PGJ 2 augmented the binding of IgG antibodies from sera containing anti-U 1 RNP and anti-SS-A/Ro antibodies to cultured keratinocytes, but produced no enhancement of the binding of IgG antibodies from sera containing anti-Sm or anti-DNA antibodies. Similar results were also obtained by using flow cytometry analysis. HSP was also induced by ultraviolet B irradiation. These results suggest that exposure of keratinocytes to stressors such as Δ 12 - PGJ 2 and ultraviolet light increases the binding sites for U 1 RNP, SS-A/Ro, and SS-B/La antibodies. The association between HSP induction and the appearance of extractable nuclear antigens may provide a better understanding of why environmental stimuli can promote the development of erythematous lesions in the skin. |
| Databáze: | OpenAIRE |
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