In-vitro degradation of Czapek and molasses amended post-harvest sugarcane residue by lignocellulolytic fungal strains
| Autor: | Marianela Maza, Hipólito F. Pajot, Marta Graciela Yasem, Maria Julia del R. Amoroso |
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| Rok vydání: | 2015 |
| Předmět: |
DECOMPOSITION
Crop residue Otras Ciencias Biológicas Cellulase MOLASSES Microbiology Ciencias Biológicas Biomaterials LACCASE Bioenergy Food science GROWTH MODEL Waste Management and Disposal Mycelium Laccase biology Chemistry business.industry POST-HARVEST SUGARCANE RESIDUE Enzyme assay Biotechnology Solid-state fermentation SOLID-STATE FERMENTATION biology.protein Fermentation business CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | International Biodeterioration & Biodegradation. 104:118-122 |
| ISSN: | 0964-8305 |
| Popis: | Post-harvest sugarcane residue (SCR), deposited on sugarcane fields after green harvesting, could serveas a substrate for fungal biomass and lignocellulolytic enzymes production. In the present study, themycelial growth of six strains (Trametes sp. YeH11, Bjerkandera sp. Y-HHM2, Phanerochaete sp. Y-RN1,Pleurotus sp. Y-RN3, Myrothecium sp. S-3.20 and Hypocrea nigricans SCT-4.4) was measured in-vitro byapplying a modified Gompertz equation. In-vitro assays showed shorter lag phases for fungi in modifiedCzapek, 0.3% and 1.0% molasses amended post-harvest SCR. Further increments in molasses concentrationsproduced a reduction on the specific growth rates for all tested fungi. Fungal degradation ofpost-harvest SCR and the concomitant enzyme production were tested under solid-state fermentation(SSF) of Czapek or molasses amended post-harvest SCR. Under SSF, Pleurotus sp. Y-RN3 produced thehighest laccase titers but no hydrolytic activity could be detected. Trametes sp. YeH11 and Myrotheciumsp. S-3.20 showed high endoglucanase activities. Endoxylanase production was detected exclusively inCzapek amended media. These findings have implications for the fungal treatment of post-harvest SCRand its potential impact on the use of these residues in the production of biofuels and ligninolyticenzymes. Fil: Maza, Marianela. Universidad Nacional de Tucuman. Facultad de Agronomia y Zootecnia. Sanidad Vegetal. Cátedra Fitopatologia; Argentina Fil: Pajot, Hipolito Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina Fil: Amoroso, Maria Julia del R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Yasem, Marta Graciela. Universidad Nacional de Tucuman. Facultad de Agronomia y Zootecnia. Sanidad Vegetal. Cátedra Fitopatologia; Argentina |
| Databáze: | OpenAIRE |
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