Diabetic Bone Marrow Cell Injection Accelerated Acute Pancreatitis Progression
| Autor: | Ji-Ming Yin, Cen Yan, Ling-Jia Meng, Guo-Tao Lu, Xiao-Min Luo, Ying-Mei Feng, Yue-Jie Zhang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
musculoskeletal diseases
Male medicine.medical_specialty Necrosis Article Subject Immunology Inflammation Bone Marrow Cells behavioral disciplines and activities Diabetes Complications Mice Internal medicine Diabetes mellitus medicine Acinar cell Immunology and Allergy Animals Humans Pancreas Bone Marrow Transplantation biology business.industry musculoskeletal neural and ocular physiology General Medicine RC581-607 medicine.disease musculoskeletal system humanities Disease Models Animal medicine.anatomical_structure Endocrinology Pancreatitis Myeloperoxidase biology.protein Disease Progression Acute pancreatitis Cytokines Bone marrow Immunologic diseases. Allergy medicine.symptom business Ceruletide Injections Intraperitoneal Research Article |
| Zdroj: | Journal of Immunology Research Journal of Immunology Research, Vol 2021 (2021) |
| ISSN: | 2314-7156 |
| Popis: | Acute pancreatitis (AP) is one of the leading causes of hospital admission, 20% of which could progress to the severe type with extensive acinar cell necrosis. Clinical studies have reported that diabetes is an independent risk factor of the incidence of AP and is associated with higher severity than nondiabetic subjects. However, how diabetes participates in AP progression is not well defined. To investigate this question, wild-type (wt) and diabetic db/db mice at the age of 16 weeks were used in the study. AP was induced in wt recipients by 10 injections of 50 μg/kg caerulein with a 1 h interval. One hour after the last caerulein injection, bone marrow cells (BMC) isolated from wt and db/db mice were injected intraperitoneally into the recipients ( 1 × 10 7 cells/recipient). The recipients with no BMC injection served as controls. Thirteen hours after BMC injection, serum lipase activity was 1.8- and 1.3-folds higher in mice that received db/db BMC, compared with those with no injection and wt BMC injection, respectively ( p ≤ 0.02 for both). By H&E staining, the overall severity score was 14.7 for no cell injection and 16.6 for wt BMC injection and increased to 22.6 for db/db BMC injection ( p ≤ 0.002 for both). In particular, mice with db/db BMC injection developed more acinar cell necrosis and vacuolization than the other groups ( p ≤ 0.03 for both). When sections were stained with an antibody against myeloperoxidase (MPO), the density of MPO+ cells in pancreatitis was 1.9- and 1.6-folds higher than wt BMC and no BMC injection groups, separately ( p ≤ 0.02 for both). Quantified by ELISA, db/db BMC produced more IL-6, GM-CSF, and IL-10 compared with wt BMC ( p ≤ 0.04 for all). In conclusion, BMC of db/db mice produced more inflammatory cytokines. In response to acinar cell injury, diabetic BMC aggravated the inflammation cascade and acinar cell injury, leading to the progression of acute pancreatitis. |
| Databáze: | OpenAIRE |
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