Wound epithelialization deficits in the transforming growth factor-alpha knockout mouse

Autor:	Injoong Kim, Jerome D. Chao, Thomas A. Mustoe, Jon E. Mogford
Rok vydání:	2001
Předmět:	Pathology medicine.medical_specialty TGF alpha Time Factors Wounds Penetrating Dermatology Epithelium Mice In vivo Head model Wound Epithelialization medicine Animals Skin Mice Knockout Wound Healing integumentary system business.industry Granulation tissue Anatomy Transforming Growth Factor alpha Disease Models Animal medicine.anatomical_structure Knockout mouse Granulation Tissue Surgery Wound edge business
Zdroj:	Wound Repair and Regeneration. 9:386-390
ISSN:	1524-475X 1067-1927
DOI:	10.1046/j.1524-475x.2001.00386.x
Popis:	In vitro, transforming growth factor-alpha is an important factor controlling epithelial cell proliferation and migration. However, the transforming growth factor-alpha knockout mouse has shown no wound epithelialization defect in tail amputation and full-thickness back wounds. To resolve this disparity, we combined a full-thickness head wound and a partial-thickness ear wound on the transforming growth factor-alpha knockout mouse for analysis of wound epithelialization with or without granulation tissue formation. Three-millimeter ear wounds were made on the transforming growth factor-alpha knockout and heterozygous control mice. Full-thickness head wounds were made using a 6-mm trephine on the crown of the skull. In the ear model, transforming growth factor-alpha knockout mice had significantly larger epithelial gaps versus control at post-operative day 3 and 5. Epithelial thickness at the wound edge of transforming growth factor-alpha deficient mice was also depressed at post-operative day 3 and post-operative day 5 compared to control mice. On post-operative day 8, most wounds of both groups were epithelialized. In contrast, no difference in epithelial gap or new granulation tissue was found in the head model. The data support the concept that transforming growth factor-alpha plays a significant early role in wound epithelialization in vivo but its deficit is compensated if accompanied by granulation tissue formation. The data further show the importance of appropriate wound models to address the role of vulnerary factors.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5bcea1c6459657a7f1655e7ea61ac30f https://doi.org/10.1046/j.1524-475x.2001.00386.x Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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