Recovery of nanogram quantities of DNA from plasma and quantitative measurement using labeling by nick translation
| Autor: | Gilbert J. Fournié, Jean J. Conté, Martine Gayral-Taminh, Jean-Pierre Bouché |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Lipopolysaccharides
food.ingredient Biophysics Biochemistry Gelatin Polyethylene Glycols Mice chemistry.chemical_compound food Blood plasma Animals Nick translation Molecular Biology Ethanol precipitation Chromatography Chemistry Microchemistry Temperature DNA Cell Biology Plasma Kinetics Nucleic acid Molecular probe |
| Zdroj: | Analytical Biochemistry. 158:250-256 |
| ISSN: | 0003-2697 |
| Popis: | A method which allows for the quantitative measurement of DNA in plasma is described. After treatment of plasma with phenol, DNA is precipitated by ethanol using gelatin as a coprecipitating agent. DNA is then measured by nick translation labeling. This assay takes a few hours. It is suitable for the measurement of DNA within a range of 0.02 to 20 ng in 10 μl of plasma. For example, it is applied to the measurement of DNA in plasma from mice injected with bacterial lipopolysaccharide. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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