Cohnella endophytica sp. nov., a novel endophytic bacterium isolated from bark of Sonneratia apetala
| Autor: | Ling-Jie Meng, Xiao-Rui Yan, Li Tuo |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine DNA Bacterial China Peptidoglycan 010603 evolutionary biology 01 natural sciences Microbiology 03 medical and health sciences Pigment RNA Ribosomal 16S Botany Ecology Evolution Behavior and Systematics Mycelium Phospholipids Phylogeny Lythraceae Bacillales Base Composition Strain (chemistry) biology Phylogenetic tree Fatty Acids Vitamin K 2 General Medicine Sequence Analysis DNA biology.organism_classification 16S ribosomal RNA Terpenoid Bacterial Typing Techniques 030104 developmental biology visual_art visual_art.visual_art_medium Plant Bark Bark Bacteria |
| Zdroj: | International journal of systematic and evolutionary microbiology. 69(7) |
| ISSN: | 1466-5034 |
| Popis: | A Gram-stain-positive, aerobic, rod-shaped, endospore-forming bacterium, designated strain M2MS4P-1T, was isolated from surface-sterilized bark of Sonneratiaapetala sampled in Guangxi, China. The bacterium was characterized by a polyphasic approach to determine its taxonomic position. 16S rRNA gene sequence comparisons revealed that strain M2MS4P-1T belonged to the genus Cohnella and was most closely to Cohnella luojiensis HY-22RT (98.4 % similarity). The average nucleotide identity value and estimated DDH value between strain M2MS4P-1T and the type strain of C. luojiensis HY-22RT were 79.2 and 20.1 %, respectively. Neither substrate nor aerial mycelia were formed, and no diffusible pigments were observed on the media tested. Strain M2MS4P-1T grew in the pH range 6.0–9.0 (optimum, pH 7.0–8.0), at temperatures between 10–37 °C (30 °C) and in 0–1 % (w/v) NaCl (0 %). The predominant isoprenoid quinone in strain M2MS4P-1T was menaquinone-7. The major fatty acids were anteiso-C15 : 0 and iso-C16 : 0. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, lysyl-phosphatidylglycerol, four unidentified aminophospholipids and two unidentified phospholipids. The DNA G+C content was 51.5 mol%. According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain M2MS4P-1T was clearly distinguishable from other species with validly published names in the genus Cohnella and should therefore be classified as a novel species, for which we suggest the name Cohnella endophytica sp. nov. The type strain is M2MS4P-1T (=KCTC 43011T=CGMCC 1.13745T). |
| Databáze: | OpenAIRE |
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