Positron-Emission Tomography Imaging of Early Events after Transplantation of Islets of Langerhans
| Autor: | Daniel O. Slosman, Axel Andres, Leo Buhler, Domenico Bosco, Habib Zaidi, Mathieu Pierre Jean Armanet, Nadine Pernin, Reto M. Baertschiger, Thierry Berney, Christian Toso, Philippe Morel |
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| Rok vydání: | 2005 |
| Předmět: |
Male
endocrine system medicine.medical_specialty endocrine system diseases Cell Islets of Langerhans Transplantation ddc:616.0757 Incubation period Rats Sprague-Dawley Islets of Langerhans Fluorodeoxyglucose F18 In vivo Internal medicine medicine Animals Tissue Distribution Transplantation geography geography.geographical_feature_category ddc:617 medicine.diagnostic_test business.industry Fdg uptake Fluorodeoxyglucose F18/diagnostic use/pharmacokinetics Islet Rats Islets of Langerhans Transplantation/physiology Radiopharmaceuticals/diagnostic use/pharmacokinetics Endocrinology medicine.anatomical_structure Positron emission tomography Positron-Emission Tomography Islets of Langerhans/radionuclide imaging Radiopharmaceuticals business Ex vivo |
| Zdroj: | Transplantation, Vol. 79, No 3 (2005) pp. 353-5 |
| ISSN: | 0041-1337 |
| DOI: | 10.1097/01.tp.0000149501.50870.9d |
| Popis: | The aim of our study was to assess cell trafficking and early events after intraportal islet transplantation. Sprague-Dawley rat islets were incubated for various times, with various concentrations of 2-[F]fluoro-2deoxy-D-glucose (FDG), and in presence of various glucose concentrations. FDG-labeled syngeneic islets or FDG alone were injected in rats. Radioactivity was measured in the liver and in various organs by positron-emission tomography for 6 hours. FDG uptake increased with incubation time or FDG concentration and decreased in presence of glucose. In vivo, all islets implanted in the liver, with an uptake 4.4 times higher than controls (44.2% vs. 10.1%, P=0.02). Radioactivity in the liver decreased at the same rate after injection of labeled-islets and FDG alone. Ex vivo labeling of islets and imaging of posttransplant early events were feasible. Islets engrafted exclusively in the liver. No islet loss could be demonstrated 6 hours after transplantation. |
| Databáze: | OpenAIRE |
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