Inhibition of Generation of Cytotoxic T Lymphocyte Activity by a CCL19/Macrophage Inflammatory Protein (MIP)-3β Antagonist
| Autor: | Shaun R. McColl, Ian Clark-Lewis, Katherine R. Pilkington |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Receptors
CCR7 Chemokine Time Factors Molecular Sequence Data Priming (immunology) C-C chemokine receptor type 7 Ligands Biochemistry Mice Immune system Cell Movement Leukocytes medicine Animals Humans Cytotoxic T cell Amino Acid Sequence Molecular Biology Inflammation Mice Inbred BALB C Chemokine CCL21 Dose-Response Relationship Drug biology Chemotaxis CCL19 CCL18 Dendritic Cells Cell Biology medicine.disease Protein Structure Tertiary Transplant rejection Cell biology Chemokines CC Immune System Mutation biology.protein Chemokine CCL19 Calcium Female Receptors Chemokine Chemokines Peptides Spleen Protein Binding T-Lymphocytes Cytotoxic |
| Zdroj: | Journal of Biological Chemistry. 279:40276-40282 |
| ISSN: | 0021-9258 |
| Popis: | Chemokines constitute a group of over 40 secreted peptides that are important for the control of leukocyte migration both during homeostasis and inflammation. Recent studies have implicated the ligands CCL19 and CCL21 and their receptor, CCR7, in the specific migration of naïve lymphocytes and mature dendritic cells to secondary lymphoid organs during immune homeostasis. However, the role that these molecules play during immune priming is not well understood. In this study, using CCL19((8-83)), a novel N-terminal truncation mutant, we have investigated the role of CCL19 in a primary allogeneic immune response, a response of particular relevance to transplant rejection. This antagonist specifically inhibited wild type CCL19-induced chemotaxis and intracellular calcium mobilization without affecting that of CCL21. The treatment of mice with CCL19((8-83)) did not globally inhibit the recruitment of cells into lymph nodes; however, it inhibited the generation of cytotoxic T lymphocytes toward allogeneic dendritic cells. This is the first evidence that CCL19 plays a role in immune priming. |
| Databáze: | OpenAIRE |
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