An adhesome comprising laminin, dystroglycan and myosin IIA is required during notochord development in Xenopus laevis
| Autor: | Elsa Denker, Thierry Darribère, Nicolas Buisson, Nicole Moreau, Aline Goullancourt, Ronan Le Bouffant, Valérie Bello, Cathy Sirour |
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| Přispěvatelé: | Laboratoire de Biologie du Développement (LBD), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), ARC, AFM, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Proteomics
Organogenesis [SDV]Life Sciences [q-bio] Blotting Western Notochord Morpholinos Xenopus laevis 03 medical and health sciences 0302 clinical medicine Tandem Mass Spectrometry Laminin medicine Dystroglycan Animals Immunoprecipitation Dystroglycans Cytoskeleton Molecular Biology [SDV.BDD]Life Sciences [q-bio]/Development Biology In Situ Hybridization ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences biology Adhesome Nonmuscle Myosin Type IIA Notochord morphogenesis Cell Polarity Cortical actin cytoskeleton Mediolateral intercalation Cell Biology Anatomy Molecular biology Cell biology medicine.anatomical_structure Bromodeoxyuridine Gene Knockdown Techniques Vacuoles biology.protein Pikachurin 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Development (Cambridge, England) Development (Cambridge, England), Company of Biologists, 2014, pp.4569-4579. ⟨10.1242/dev.116103⟩ Development (Cambridge, England), 2014, pp.4569-4579. ⟨10.1242/dev.116103⟩ |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.116103⟩ |
| Popis: | Dystroglycan (Dg) is a transmembrane receptor for laminin that must be expressed at the right time and place in order to be involved in notochord morphogenesis. The function of Dg was examined in Xenopus laevis embryos by knockdown of Dg and overexpression and replacement of the endogenous Dg with a mutated form of the protein. This analysis revealed that Dg is required for correct laminin assembly, for cell polarization during mediolateral intercalation and for proper differentiation of vacuoles. Using mutations in the cytoplasmic domain, we identified two sites that are involved in cell polarization and are required for mediolateral cell intercalation, and a site that is required for vacuolation. Furthermore, using a proteomic analysis, the cytoskeletal non-muscle myosin IIA has been identified for the first time as a molecular link between the Dg-cytoplasmic domain and cortical actin. The data allowed us to identify the adhesome laminin-Dg-myosin IIA as being required to maintain the cortical actin cytoskeleton network during vacuolation, which is crucial to maintain the shape of notochordal cells. |
| Databáze: | OpenAIRE |
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