Effect of uterine ozone therapy and anticoagulant sampling on oxidative stress parameters in mares
Autor: | Letícia Ramos Costa, Lidiana Zanetti Amatti, Fernanda Saules Ignácio, Daniela Fernandez Montechiesi, Beatriz Perez Floriano, Paula Lima de Oliveira, Jean Guilherme Fernandes Joaquim, Luana Venâncio Garcia, Camila Peres Rubio, Maria Rachel Melo Bosculo, Breno Fernando Martins de Almeida, Giovanna Gati de Souza |
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Rok vydání: | 2020 |
Předmět: |
medicine.medical_specialty
medicine.drug_class Uterus medicine.disease_cause Gastroenterology Antioxidants Lipid peroxidation chemistry.chemical_compound Ozone Internal medicine medicine Animals Horses General Veterinary business.industry Anticoagulant Albumin Anticoagulants Heparin Ozone therapy Uric Acid Oxidative Stress medicine.anatomical_structure chemistry Uric acid Female Lipid Peroxidation business Oxidative stress Biomarkers medicine.drug |
Zdroj: | Research in veterinary science. 136 |
ISSN: | 1532-2661 |
Popis: | With the purpose of assessing the effects of uterine ozone therapy and anticoagulant sampling on oxidative stress (OS) parameters in mares, ten mares underwent three consecutive days of uterine ozone therapy by flushing the uterus with ozonated lactated Ringer's solution followed by insufflation with ozone‑oxygen gas. Serum samples were obtained at baseline and days 3, 6, 10 and 17 to determine the effect of ozone therapy on OS markers. Plasma obtained with anticoagulants citrate, ethylenediaminetetraacetic acid (EDTA) and heparin were at baseline and 6 days following therapy to determine the effect of anticoagulant on OS parameters. Antioxidants albumin and uric acid, total antioxidant capacity (TAC) using four different methods, total oxidant capacity (TOC) and lipid peroxidation were determined through photocolorimetry. Statistical analyses comprised repeated measures ANOVA followed by Dunnett's test or Friedman followed by Dunn's post-hoc test. Differences were considered significant when p 0.05. Uterine ozone therapy significantly decreased uric acid, TAC in all four different methods, concomitantly with an increase on TOC at days 3 and 6 following therapy. No changes were observed on albumin and lipid peroxidation levels. Anticoagulants prevented the detection of oxidative stress induced by uterine ozone therapy depending on the method of analysis. In conclusion, uterine ozone therapy causes systemic oxidative stress in mares and the choice of anticoagulant sampling interferes with laboratory tests. |
Databáze: | OpenAIRE |
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