Distinct Glycoforms of a Tumor Specific Glycoprotein, gp200, in Human Testis and Testicular Tumors

Autor:	William C. DeWolf, Mireille Gregoire, William M. Schopperle
Rok vydání:	1995
Předmět:	Peanut agglutinin chemistry.chemical_classification medicine.medical_specialty biology Somatic cell Urology Lectin Seminoma medicine.disease Molecular biology Embryonal carcinoma Endocrinology chemistry Cell culture Internal medicine medicine biology.protein Germ cell tumors Glycoprotein
Zdroj:	Journal of Urology. 154:275-277
ISSN:	1527-3792 0022-5347
Popis:	We recently purified a 200 kD peanut agglutinin-binding glycoprotein from the embryonal carcinoma cell line HT-E (833K) that is expressed selectively in nonseminomatous germ cell tumors. We now further characterize gp200 using Western blot analysis to compare normal and malignant testicular germ cells for reactivity to peanut agglutinin, a plant lectin that recognizes terminal D-galactosyl residues, and GCTM-2, a monoclonal antibody that recognizes a 200 kD keratan-sulfate proteoglycan on human embryonal carcinoma. The results indicate that normal germ cells express gp200 as a membrane-bound 230 kD glycoform that expresses terminal galactose residues. This 230 kD glycoprotein is absent on spermatozoa but present on seminomatous germ cell tumors and somatic tissue and does not express terminal galactose on its carbohydrate side chains. In contrast, nonseminomatous germ cell tumors express a heavily sialylated glycoform of gp200 that does express terminal galactose residues. These results describe a...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5bc27b3b864150ad1d797f2fbc808ea8 https://doi.org/10.1016/s0022-5347(01)67293-8 Zobrazit plný text záznamu