Decreasing Effect of Lidocaine·HCl on the Thickness of the Neuronal and Model Membrane
| Autor: | Jae-Han Kim, Do-Keun Lee, Jin-Hyun Baek, Il Yun, Hye-Ock Jang, Tae-Gyun Yoon, In-Kyo Chung, Jong Sun Park, Won-Hyang Ryu, Sung-Min Park, Uy Dong Sohn |
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| Rok vydání: | 2013 |
| Předmět: |
Pharmacology
medicine.medical_specialty Liposome Membrane thickness Physiology Chemistry Arbitrary unit Fluorescence Surgery Membrane medicine.anatomical_structure Mechanism of action Lidocaine·HCl Cerebral cortex Neuronal membranes Anesthetic medicine Biophysics Model membranes Original Article Fluorescence quenching technique medicine.symptom Lipid bilayer medicine.drug |
| Zdroj: | The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology |
| ISSN: | 2093-3827 1226-4512 |
| DOI: | 10.4196/kjpp.2013.17.4.253 |
| Popis: | This study examined the mechanism of action of a local anesthetic, lidocaine·HCl. Energy transfer between the surface fluorescent probe, 1-anilinonaphthalene-8-sulfonic acid, and the hydrophobic fluorescent probe, 1,3-di(1-pyrenyl) propane, was used to determine the effect of lidocaine·HCl on the thickness (D) of the synaptosomal plasma membrane vesicles (SPMV) isolated from the bovine cerebral cortex, and liposomes of the total lipids (SPMVTL) and phospholipids (SPMVPL) extracted from the SPMV. The thickness (D) of the intact SPMV, SPMVTL and SPMVPL were 1.044±0.008, 0.914±0.005 and 0.890±0.003 (arbitrary units, n=5) at 37℃ (pH 7.4), respectively. Lidocaine·HCl decreased the thickness of the neuronal and model membrane lipid bilayers in a dose-dependent manner with a significant decrease in the thickness, even at 0.1 mM. The decreasing effect of lidocaine·HCl on the membrane thickness might be responsible for some, but not all of its anesthetic action. |
| Databáze: | OpenAIRE |
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