Polyphosphate kinases modulateCampylobacter jejuniouter membrane constituents and alter its capacity to invade and survive in intestinal epithelial cellsin vitro
| Autor: | Ruby Pina-Mimbela, Anand Kumar, Jesús Arcos Madrid, Gireesh Rajashekara, Jordi B. Torrelles |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Epidemiology
Immunology In Vitro Techniques survival Microbiology Campylobacter jejuni Bacterial cell structure Cell Line 03 medical and health sciences Polyphosphate kinase Immune system Anti-Infective Agents Virology Campylobacter Infections Drug Discovery Humans Molecular Targeted Therapy poly P kinases 030304 developmental biology 0303 health sciences Phosphotransferases (Phosphate Group Acceptor) biology outer membrane constituents 030306 microbiology Kinase Interleukin-8 Epithelial Cells General Medicine invasion biology.organism_classification Gastroenteritis 3. Good health Infectious Diseases Interaction with host Original Article Parasitology Bacterial outer membrane Intracellular |
| Zdroj: | Emerging Microbes & Infections |
| ISSN: | 2222-1751 |
| DOI: | 10.1038/emi.2015.77 |
| Popis: | Campylobacter jejuni is the most prevalent cause of bacterial gastroenteritis worldwide. Polyphosphate kinases 1 and 2 (PPK1 and PPK2) regulate several cellular processes, including the biosynthesis of the bacterial cell wall. Despite their importance, whether PPK1 and PPK2 modulate the composition of C. jejuni outer membrane constituents (OMCs) and consequently impact its interaction with host cells remains unknown. Our comparative analysis between C. jejuni wild type, Δppk1, and Δppk2 strains showed qualitative and quantitative differences in the total OMC composition among these strains. Importantly, these OMC variations observed on the C. jejuni polyphosphate kinase mutants are directly related to their capacity to invade, survive, and alter the immune response of intestinal epithelial cells in vitro. Specifically, sub-fractionation of the C. jejuni OMC indicated that OMC proteins are uniquely associated with bacterial invasion, whereas C. jejuni OMC proteins, lipids, and lipoglycans are all associated with C. jejuni intracellular survival. This study provides new insights regarding the function of polyphosphate kinases and their role in C. jejuni infection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|