Type 2 rhinovirus infection of cultured human tracheal epithelial cells: role of LDL receptor
| Autor: | Katsutoshi Nakayama, Norihiro Yamada, Hidekazu Nishimura, Tomoko Suzuki, Hidetada Sasaki, Masahito Kamanaka, Mutsuo Yamaya, Yu X. Jia, Masayoshi Hosoda, Kiyohisa Sekizawa |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine Rhinovirus Sp1 Transcription Factor Physiology Common Cold Inflammation Biology medicine.disease_cause Polymerase Chain Reaction Antibodies Microbiology Physiology (medical) medicine Humans Receptor Cells Cultured Aged Tumor Necrosis Factor-alpha NF-kappa B Epithelial Cells DNA Cell Biology Middle Aged Virology Epithelium Trachea medicine.anatomical_structure Receptors LDL LDL receptor biology.protein Cytokines RNA Viral Female Tumor necrosis factor alpha Disease Susceptibility medicine.symptom Antibody Interleukin-1 Lipoprotein |
| Zdroj: | American Journal of Physiology-Lung Cellular and Molecular Physiology. 280:L409-L420 |
| ISSN: | 1522-1504 1040-0605 |
| DOI: | 10.1152/ajplung.2001.280.3.l409 |
| Popis: | To examine the role of the low-density lipoprotein (LDL) receptor on minor group human rhinovirus (RV) infection, primary cultures of human tracheal epithelial cells were infected with a minor group (RV2) or a major group (RV14) RV. Viral infection was confirmed by showing with PCR that viral titers in supernatants and lysates from infected cells increased with time. RV2 and RV14 increased expression of mRNA and protein of the LDL receptor on the cells and the cytokine production. RV2 induced activation of transcription factors SP1 and nuclear factor-κB (NF-κB). An antibody to the LDL receptor inhibited RV2 infection and RV2-induced cytokine production without an effect on RV14 infection and RV14-induced cytokine production. These findings imply that RV2 upregulates LDL receptor expression on airway epithelial cells, thereby increasing susceptibility to minor group RV infection. LDL receptor expression and cytokine production may be mediated, in part, via activation of transcription factors by RV2. These events may be important in airway inflammation after minor group RV infection in asthma. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|