Ultrastructural GABA immunogold labeling in the substantia nigra pars reticulata of kindled genetic absence epilepsy rats

Autor:	Dilek Akakin, Filiz Onat, Serap Sirvanci, Özlem Tuğçe Çilingir Kaya, Zehra Turgan Aşik, Tugba Karamahmutoglu, Medine Gulcebi Idrizoglu
Rok vydání:	2020
Předmět:	Male 0301 basic medicine medicine.medical_specialty Immunocytochemistry Stimulation Biology Amygdala Pathology and Forensic Medicine Synapse 03 medical and health sciences Epilepsy 0302 clinical medicine Microscopy Electron Transmission Structural Biology Internal medicine Pars Reticulata Kindling Neurologic medicine Animals Rats Wistar gamma-Aminobutyric Acid Kindling Immunogold labelling medicine.disease Immunohistochemistry Rats Disease Models Animal 030104 developmental biology Endocrinology medicine.anatomical_structure Epilepsy Absence 030220 oncology & carcinogenesis Synapses Ultrastructure
Zdroj:	Ultrastructural Pathology. 44:379-386
ISSN:	1521-0758 0191-3123
DOI:	10.1080/01913123.2020.1839153
Popis:	Genetic Absence Epilepsy Rats from Strasbourg (GAERS) is a well-known animal model of absence epilepsy and they are resistant to electrical kindling stimulations. The present study aimed to examine possible differences in gamma-aminobutyric acid (GABA) levels and synapse counts in the substantia nigra pars reticulata anterior (SNRa) and posterior (SNRp) regions between GAERS and Wistar rats receiving kindling stimulations. Animals in the kindling group either received six stimulations in the amygdala and had grade 2 seizures or they were kindled, having grade five seizures. Rats were decapitated one hour after the last stimulation. SNR regions were obtained after vibratome sectioning of the brain tissue. GABA immunoreactivity was detected by immunogold method and synapses were counted. Sections were observed by transmission electron microscope and analyzed by Image J program. GABA density in the SNRa region of fully kindled GAERS and Wistar groups increased significantly compared to that of their corresponding grade 2 groups. The number of synapses increased significantly in kindled and grade 2 GAERS groups, compared to kindled and grade 2 Wistar groups, respectively, in the SNRa region. GABA density in the SNRp region of kindled GAERS group increased significantly compared to that of GAERS grade 2 group. In the SNRp region, both kindled and grade 2 GAERS groups were found to have increased number of synapses compared to that of GAERS control group. We concluded that both SNRa and SNRp regions may be important in modulating resistance of GAERS to kindling stimulations.
Databáze:	OpenAIRE
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