PREFERENTIAL LABELING OF RAT LYMPHOCYTES WITH A RAPID RATE OF TURNOVER BY TRITIATED DEOXYCYTIDINE
| Autor: | N. B. Everett, M. Amano |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Male
Pyrimidine Lymphocyte Bone Marrow Cells Thymus Gland Deoxycytidine Cytosine Peyer's Patches chemistry.chemical_compound Bone Marrow medicine Animals Lymphocytes DNA synthesis Germinal center DNA Cell Biology General Medicine Molecular biology Rats Thymine medicine.anatomical_structure chemistry Biochemistry Autoradiography Specific activity Lymph Nodes Spleen Thymidine |
| Zdroj: | Cell Proliferation. 9:167-177 |
| ISSN: | 1365-2184 0960-7722 |
| DOI: | 10.1111/j.1365-2184.1976.tb01264.x |
| Popis: | Lymphocytes in thymic cortex and germinal centers of lymphoid tissues are labeled intensely with generally labeled tritiated deoxycytidine [G-3H]dCyd whereas they are weakly labeled with methyl tritiated deosythymidine [methyl3H]dThd of the same specific activity, not only by single injection but also by an intensive injection schedule. [G-3H]dCyd can be used to label short-lived lymphocytes strongly, although not specifically. The distribution patterns of labeled lymphocytes were different depending on the injection schedules of [G-3H]dCyd. [G-3H]dCyd can be used as a precursor molecule for cytosine and also thymine found in DNA. The ratios of radioactive thymine to crytosine measured biochemically on DNA extracted from radioactive lymphocytes labeled by the various schedules indicate strongly that short- and long-lived lymphocyte populations have different abilities to utilize pyrimidine nucleosides for DNA synthesis. |
| Databáze: | OpenAIRE |
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