Empirical evaluation of DArT, SNP, and SSR marker-systems for genotyping, clustering, and assigning sugar beet hybrid varieties into populations

Autor: Theo van Hintum, Imad A. Eujayl, Ivan Simko
Rok vydání: 2012
Předmět:
Genetic Markers
Centre for Genetic Resources
The Netherlands
Linkage disequilibrium
Genotyping Techniques
Population Dynamics
Statistics as Topic
Population
beta-vulgaris l
Centre for Genetic Resources
Single-nucleotide polymorphism
Locus (genetics)
Plant Science
Biology
Polymorphism
Single Nucleotide
simple sequence repeat
map
Genetics
Cluster Analysis
education
genome
Genotyping
inference
Genetic diversity
education.field_of_study
business.industry
food and beverages
The Netherlands
genetic diversity
General Medicine
microsatellite markers
Biotechnology
Genetics
Population
Centrum Genetische Bronnen Nederland
Genetic Loci
Genetic marker
Area Under Curve
technology dart
Hybridization
Genetic
Microsatellite
Beta vulgaris
polymorphisms
business
human activities
Agronomy and Crop Science
linkage disequilibrium
Microsatellite Repeats
Zdroj: Plant Science, 184, 54-62
Plant Science 184 (2012)
ISSN: 0168-9452
DOI: 10.1016/j.plantsci.2011.12.009
Popis: Dominant and co-dominant molecular markers are routinely used in plant genetic research. In the present study we assessed the success-rate of three marker-systems for estimating genotypic diversity, clustering varieties into populations, and assigning a single variety into the expected population. A set of 54 diploid sugar beet (Beta vulgaris L. ssp. vulgaris) hybrid varieties from five seed companies was genotyped with 702 Diversity Array-Technology (DArT), 34 Single Nucleotide Polymorphisms (SNP), and 30 Simple Sequence Repeats (SSR) markers. Analysis of the population structure revealed three well-defined populations and clustering of varieties that generally correlates with their seed company origin. Two populations each contained varieties from two different seed companies indicating genetic similarity of this material. The third population was comprised only of varieties from a single seed company. Analysis of the SSR and SNP datasets indicates that some of the hybrid varieties likely have a common (or very closely related) parent. Comparison of the three marker-systems revealed substantial differences in the number of loci needed for analyses. Varietal clustering required approximately 1.8–2 × more SSR, 3–4.5 × more SNP, and 4.8 × more DArT markers than were required for detection of genotypic diversity. When marker-systems were compared across different types of analyses per locus success-rate was the highest for the SSR and the lowest for the DArT markers. Generally, about 1.4–3 × more SNPs, and 4.9–13.3 × more DArTs then SSRs were needed to achieve the 100% success-rate. However, using only DArT markers with a high level of polymorphism decreased the number of DArT loci needed for analyses by 38–61%. Results from the present work provide a premise to selecting the type(s) and number of markers that are needed for genetic diversity analysis of sugar beet hybrid varieties
Databáze: OpenAIRE
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