Expression of nitric oxide synthases and in vitro migration of eosinophils from allergic rhinitis subjects
| Autor: | Antonio F. Salaroli, Antonio Roberto Martins, Nicola Conran, Heloisa H.A. Ferreira, Edson Antunes, Gilberto De Nucci, Mônia L.S. Lodo, Ludmyla Kandratavicius |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Eotaxin
Gene isoform Chemokine CCL11 medicine.medical_specialty Allergy Rhinitis Allergic Perennial Nitric Oxide Synthase Type II S-Nitroso-N-Acetylpenicillamine Nitric Oxide Nitric oxide chemistry.chemical_compound Cell Movement Internal medicine Quinoxalines medicine Humans Nitric Oxide Donors Enzyme Inhibitors Pharmacology Oxadiazoles biology Dose-Response Relationship Drug Chemotaxis Eosinophil medicine.disease Dibutyryl Cyclic GMP Nitric oxide synthase Eosinophils Isoenzymes N-Formylmethionine Leucyl-Phenylalanine Endocrinology medicine.anatomical_structure NG-Nitroarginine Methyl Ester chemistry Solubility Guanylate Cyclase Chemokines CC Molsidomine biology.protein Nitric Oxide Synthase |
| Zdroj: | European journal of pharmacology. 442(1-2) |
| ISSN: | 0014-2999 |
| Popis: | The expression of nitric oxide (NO) synthases and the role of the NO cyclic GMP pathway on the migration of eosinophils from untreated patients with allergic rhinitis were investigated. Inducible NO synthase was strongly expressed in eosinophils from healthy individuals, but not in eosinophils from allergic rhinitis patients. The neuronal isoform was observed in eosinophils from each group studied, whereas no staining for the endothelial isoform was detected in either group. The chemotaxis to N-formyl-methionyl-leucyl-phenylalanine (fMLP, 5×10−7 M) and eotaxin (100 ng/ml) was significantly potentiated in allergic rhinitis eosinophils. In both groups, Nω-nitro- l -arginine methyl ester ( l -NAME, 1.0 mM) or 1H(1,2,4)-oxadiazolo(4,3,-a)quinoxalin-1-one (ODQ, 0.2 mM) markedly reduced the chemotaxis. The selective iNOS inhibitor N-(3-(aminomethyl)benzyl)acetamidine (1400 W, 0.1–1.0 mM) significantly reduced the chemotaxis of eosinophils from healthy but not from allergic rhinitis subjects. The inhibition by l -NAME was restored by 3-morpholinosydnonimine (SIN-1) and S-nitroso-N-acetyl-penicillamine, whereas the inhibition by ODQ was restored by dibutyryl cyclic GMP. In conclusion, both endothelial and inducible NO synthase isoforms are absent in allergic rhinitis eosinophils, suggesting that the NO cyclic GMP pathway in this cell type is maintained through the activity of a neuronal isoform. |
| Databáze: | OpenAIRE |
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